Hybrid Langmuir-Blodgett film of carbon nanotubes and algal polysaccharides to immobilize enzymes

• Grant number: 16/01867-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2016
• Effective date (End): December 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Raul Torres Rodrigues
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

This project aims at studying the action of carbon nanotubes and algal polysaccharides as mediators for the immobilization of enzymes as Langmuir and Langmuir-Blodgett (LB) films constituted of lipids, which serve as bioinspired systems. Lipids of various kinds will be used, among the candidates are the dipalmitoyl phosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG) and dioctadecyl dimethylammonium (DODAB), which will be co-spread on the air-water interface with the enzyme urease. The incorporation of carbon nanotubes and algal polysaccharides, or via the aqueous subphase or via immersion of pre-prepared film in the solution / dispersion will be evaluated in order to improve the optical and electric signals in the detection of the enzymatic activity. The incorporation of the enzyme in the air-water interface in the mixed lipid system / polysaccharide / nanotube is measured by surface pressure and surface potential measurements, kinetic curves adsorption, compression isotherms, microscopy Brewster angle (BAM,) and polarization-modulation reflection-absorption infrared spectroscopy (PM-IRRAS). The films will be transferred to solid supports using the Langmuir-Blodgett technique and characterized by spectroscopic techniques. The results will be evaluated from the perspective of possible implications in nanobiotechnology, inferred from the effects caused by carbon nanotubes and algal polysaccharides in artificial membranes, especially as the incorporation of enzymes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, RAUL T.; MORAIS, PAULO V.; NORDI, CRISTINA S. F.; SCHOENING, MICHAEL J.; SIQUEIRA, JR., JOSE R.; CASELI, LUCIANO. Carbon Nanotubes and Algal Polysaccharides To Enhance the Enzymatic Properties of Urease in Lipid Langmuir-Blodgett Films. Langmuir, v. 34, n. 9, p. 3082-3093, MAR 6 2018. (15/23446-0, 16/01867-6)

RODRIGUES, RAUL TORRES; SOUZA NORDI, CRISTIANA FREIRE; SIQUEIRA JUNIOR, JOSE ROBERTO; CASELI, LUCIANO. Effect of interfering agents for urease immobilized in Langmuir-Blodgett films of controlled molecular architecture. Thin Solid Films, v. 704, JUN 30 2020. (19/03239-0, 16/01867-6)