Bacteria possess the ability to form structured communities, when attached to surfaces give rise to biofilms. Several factors contribute to the formation of biofilm as the type of cells present in the liquid to which the surface is exposed, the flow velocity of the liquid across the surface, the physicochemical properties of the surface, the nutritional composition of the medium, the availability of oxygenation and temperature. However, studies involving biofilms in nosocomial microenvironments such as water circuit hemodialysis services or hemodialysis machines are rarely found in the literature. This study proposes to assess the biofilm formation ability by Escherichia coli (5 strains), Pseudomonas aeruginosa (41 strains) and Staphylococcus epidermidis (11 strains) in four fluid (dialysate, dialysate with glucose, acid buffer and buffer basic) and using a standardized culture. Strains from water circuit of a Hemodialysis Service and reference strains will be used. The 96 well microtiter plate model for biofilm formation at 37 ° C for 24h followed by the crystal violet discoloration methodology will be used to determine the biomass of biofilms. The scanning electron microscopy will show the morphology and topography of biofilms, while the matrix and its thickness will be seen with the aid of a confocal laser scanning microscopy. The study can contribute with information and knowledge to integrate bacteria in the biofilms growth mode on standardization of water used in dialysis services. Currently, these standardizations are focused only on bacteria growth in free form.
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