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Influence of fluids used for renal therapy on the development of bacterial biofilms

Grant number: 16/05287-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2016
Effective date (End): December 31, 2016
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Regina Helena Pires
Grantee:Patricia Silva Bianchi Costa
Host Institution: Coordenadoria Adjunta de Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil


Bacteria possess the ability to form structured communities, when attached to surfaces give rise to biofilms. Several factors contribute to the formation of biofilm as the type of cells present in the liquid to which the surface is exposed, the flow velocity of the liquid across the surface, the physicochemical properties of the surface, the nutritional composition of the medium, the availability of oxygenation and temperature. However, studies involving biofilms in nosocomial microenvironments such as water circuit hemodialysis services or hemodialysis machines are rarely found in the literature. This study proposes to assess the biofilm formation ability by Escherichia coli (5 strains), Pseudomonas aeruginosa (41 strains) and Staphylococcus epidermidis (11 strains) in four fluid (dialysate, dialysate with glucose, acid buffer and buffer basic) and using a standardized culture. Strains from water circuit of a Hemodialysis Service and reference strains will be used. The 96 well microtiter plate model for biofilm formation at 37 ° C for 24h followed by the crystal violet discoloration methodology will be used to determine the biomass of biofilms. The scanning electron microscopy will show the morphology and topography of biofilms, while the matrix and its thickness will be seen with the aid of a confocal laser scanning microscopy. The study can contribute with information and knowledge to integrate bacteria in the biofilms growth mode on standardization of water used in dialysis services. Currently, these standardizations are focused only on bacteria growth in free form.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COSTA, P. S. B.; OLIVEIRA, L. T.; PEDROSO, R. S.; TOSTA, P. O.; MARTINS, C. H. G.; JAMUR, M. C.; PIRES, R. H.. Single-species (bacterial, fungal, or mycobacterial) biofilms or dual-species (mycobacterial-fungal) biofilms formed in dialysis fluids. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 96, n. 3, . (15/19090-5, 16/03834-8, 16/05287-4)

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