Hydrogels of amniotic membrane and poly(ethylene glycol) (PEG) to the regeneration cartilaginous tissue

• Grant number: 16/01584-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2016
• End date: February 28, 2018
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Anderson de Oliveira Lobo
• Grantee: Tatiane Venturott Toniato
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated research grant: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration, AP.JP

Abstract

Rheumatic disease is the term used to denote different diseases that have in common the commitment of bones, cartilage, joints, tendons, ligaments and/or muscles. Rheumatic diseases affect about 12 million Brazilians affecting patients physical, psychological and social, and may lead them to disability. When biological tissue is damaged or lost, there is the need to use a material that may replace it, providing similar conditions to those found inside the human body, stimulating its natural regeneration process. To these materials gives the name of biomaterials. Among the biomaterials studies demonstrate the efficient use of hydrogels for the regeneration of bone, cartilage and vasculature in addition to the controlled delivery of drugs. It is hydrated polymeric materials, generally produced by curing of monomers with the formation of crosslinks between chains. Thanks to its texture, hydrogels may be injected directly into the damaged tissue in a minimally invasive manner. A variety of natural and synthetic polymers are used for the production of hydrogels, and polyethylene glycol (PEG), a highlight material thanks to their properties of biocompatibility, low immunogenicity and solubility in water. PEG is commonly modified by the insertion of functional groups to improve their properties, including polyethylene glycol-dimethacrylate (PEGDMA) stands out thanks to its good mechanical properties. The amniotic membrane (AM) has been studied as possible in Regenerative Medicine biomaterial with good results in the treatment of corneal diseases, heart, liver and lung. However, little is said about the use of MA associated with the regeneration of cartilage tissue. Therefore, this research project aims to develop hydrogels PEGDMA MA and providing rheological characterization, morphological, thermal and sufficient degradation for future cartilaginous tissue regeneration therapy using MA. Hydrogels are also tested in vitro to assess cell viability and adhesion of human chondrocytes used. It is expected observed through tests such as the amniotic membrane hydrogel has applicability in the treatment of diseases associated with loss of cartilage tissue. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TONIATO, TATIANE VENTUROTT; STOCCO, THIAGO DOMINGUES; MARTINS, DANILO DOS SANTOS; SANTANNA, LUCIANA BARROS; TIM, CARLA ROBERTA; MARCIANO, FERNANDA ROBERTA; SILVA-FILHO, EDSON CAVALCANTI; CAMPANA-FILHO, SERGIO PAULO; LOBO, ANDERSON DE OLIVEIRA. Hybrid chitosan/amniotic membrane-based hydrogels for articular cartilage tissue engineering application. INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS, JULY 2019. (16/01584-4)

TONIATO, TATIANE VENTUROTT; STOCCO, THIAGO DOMINGUES; MARTINS, DANILO DOS SANTOS; SANTANNA, LUCIANA BARROS; TIM, CARLA ROBERTA; MARCIANO, FERNANDA ROBERTA; SILVA-FILHO, EDSON CAVALCANTI; CAMPANA-FILHO, SERGIO PAULO; LOBO, ANDERSON DE OLIVEIRA. Hybrid chitosan/amniotic membrane-based hydrogels for articular cartilage tissue engineering application. INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS, v. 69, n. 15, p. 961-970, OCT 12 2020. (16/01584-4)