The sugar-alcohol industry occupies a prominent position in Brazil. Regarding to the production of fuel ethanol, today Brazil has a demand similar to the gasoline, demonstrating the industry's greatness. Along the ethanol production occur the generating of a large amount of waste, such as vinasse, filter cake and bagasse. Among these wastes, vinasse has major environmental concerns due to its composition and disposal. Currently vinasse is used in fertigation, feeding and watering the soil of sugarcane plantations. However, the risk of rivers and ground water contamination with substances that may pose risks to the environment are huge. The substances in the vinasse that endanger the environment are antibiotics used to eliminate contamination that could harm the fermentation used in fuel ethanol production process. One of the vinasse treatment processes that has shown greater efficiency and economic viability in the removal of organic compounds is anaerobic digestion. However, the antibiotics presence in the vinasse can compromise the performance of this process. The antibiotics determination in environmental samples, and generally complex, has been successfully carried out using, among other techniques, liquid chromatography coupled mass spectrometry due to the technical characteristics. In this context, this work aims to fill some gaps in the scientific literature of the area, which are: to develop analytical methods employing liquid chromatography coupled with mass spectrometry (LC-MS / MS) for the determination of antibiotics in vinasse and treated soil with vinasse and quantitatively evaluate the effect of the presence of these antibiotics in vinasse anaerobic biodigestion.
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