Development of an electrochemical immunosensor based on lateral flow for cardiac markers detection

• Grant number: 16/08166-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2016
• Effective date (End): February 29, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Lauro Tatsuo Kubota
• Grantee: Juliana de Fátima Giarola
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/22127-2 - Development of novel materials strategic for integrated analytical devices, AP.TEM

Abstract

Cardiovascular disease is one of the most prevalent medical conditions of modern times and accounts for approximately half of all the deaths within the western world. Acute myocardial infarction is the most common form of cardiovascular disease. An accurate diagnosis and proper medical treatment are essential to increase the survival rate. This diagnosis has been done by monitoring the concentration of biomarkers such as cardiac troponin.Recent advances in the field of bioanalysis have been considerable and stimulated efforts to the development of fast, cost-effective and simplified analytical systems, primarily to diagnose diseases that affect a large part of the global population. At this point we highlight the point-of-care devices, which were developed to the rapid generation of results, being used as tools efficient at detect of a disease.The immunosensors based on lateral flow with electrochemical transduction may be employed as point-of-care technology for various applications, since they offer benefits as miniaturization and high sensitivity. This is a line of promising work to be underexplored and also because the coupling of lateral flow device for electrochemical detection provide greater sensitivity and low limits of detection for cardiac troponin, enabling early diagnosis in patients at high risk of acute myocardial infarction and increasing survival rates.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIAROLA, JULIANA DE F.; SOUTO, DENIO E. P.; KUBOTA, LAURO T.. Evaluation of PAMAM Dendrimers (G3, G4, and G5) in the Construction of a SPR-based Immunosensor for Cardiac Troponin T. Analytical Sciences, v. 37, n. 7, p. 1007-1013, JUL 2021. (14/50867-3, 16/08166-3, 13/22127-2)

PASCHOALINO, WALDEMIR J.; KOGIKOSKI, JR., SERGIO; BARRAGAN, JOSE T. C.; GIAROLA, JULIANA F.; CANTELLI, LORY; RABELO, THAIS M.; PESSANHA, TATIANA M.; KUBOTA, LAURO T.. Emerging Considerations for the Future Development of Electrochemical Paper-Based Analytical Devices. CHEMELECTROCHEM, v. 6, n. 1, SI, p. 10-30, JAN 2 2019. (17/05213-3, 16/08166-3, 16/14507-8, 13/22127-2, 14/50867-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GIAROLA, Juliana de Fátima. Study of dendrimer immobilization in the construction of a SPR-based immunosensor for detecting troponin T in early infarct diagnoses. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.