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Effects of cystic fibrosis on the oral cavity of pediatric patients

Grant number: 15/23318-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2016
Effective date (End): April 09, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Cristiane Yumi Koga Ito
Grantee:Laura Soares Souto Lepesqueur
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Associated scholarship(s):18/02725-6 - Effects of cystic fibrosis in the oral cavity pediatric patient, BE.EP.DR


Cystic fibrosis (CF) is a genetic disease with high global prevalence and that causes abnormal function of exocrine glands. Due to this compromised function, patients have several clinical manifestations related to high mortality and morbidity rates, in particular chronic infections of superior and inferior airways. Alterations in salivary glands can lead to oral impacts, however they are scarcely known. Besides of the influence of oral health on the global health condition, the oral cavity may represent a microbial reservoir for chronic pulmonary infections. For this purpose, the aim of this study is to evaluate the impact of cystic fibrosis on the oral microbiota, teeth, saliva and oral health of pediatric patients. Hundred children, aged from 6 to 12 years, divided into 3 groups will be included in the study: G1- 25 children diagnosed as cystic fibrosis, CFTR mutation detected and Shwachman-Kulczycki score between 100 and 71; G2- 25 children diagnosed as CF, CFTR mutation detected and Shwachman-Kulczycki score under 40; and G3 (control) - 50 children without diagnosis of CF, aged and gender paired to CF children. Patients with CF will be selected at Escola Paulista de Medicina (UNIFESP), while controls will be selected at ICT/ UNESP. Patients will be examined to evaluate the presence of oral lesions and caries. Oral microbiome will be analyzed by Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) for screening and by microbiological culture methodologies, aiming to evaluate the potential opportunistic and cariogenic microbiota. The impacts of CF on saliva will be evaluated by salivary proteome analyses and salivary immunoglobulin quantification. Data will be statistically analyzed using the GraphPad Prism software version 6.0 (GraphPad Software Inc., San Diego, CA, USA). In order to employ the most appropriate test, the adherence to the normal curve will be checked by a test of normality. The results will be analyzed by appropriate statistical tests, with significance level of 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUTO LEPESQUEUR, LAURA SOARES; TANAKA, MARCIA HIROMI; GOUVEA LIMA, GABRIELA DE MORAIS; CHIBA, SONIA MAYUMI; MOTA, ADOLFO JOSE; SANTOS, SAMARA FERREIRA; KOGA-ITO, CRISTIANE YUMI. Oral prevalence and antifungal susceptibility of Candida species in cystic fibrosis patients. ARCHIVES OF ORAL BIOLOGY, v. 116, AUG 2020. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LEPESQUEUR, Laura Soares Souto. Efeitos da fibrose cística sobre o microbioma bucal e o proteoma salivar. 2019. 201 f. Doctoral Thesis - Universidade Estadual Paulista "Julio de Mesquita Filho" Instituto de Ciência e Tecnologia (Campus de São José dos Campos)..

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