Physicochemical and biological characteristics of new repair materials based on tricalcium silicate and Biosilicate

• Grant number: 16/11036-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2016
• End date: March 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Mario Tanomaru Filho
• Grantee: Marcela Borsatto Queiroz
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Calcium silicate cements are developed as repair materials. Biosilicate is a bioactive glass-ceramic fully crystallized. This project is divided into two subprojects. 1- To develop and evaluate a new material based on tricalcium silicate associated with the radiopacifier calcium tungstate or niobium oxide and compare it to the MTA HP. 2 - To develop and evaluate a new material based on tricalcium silicate associated with the radiopacifier zirconium oxide and 10 or 20% of Biosilicate, and compare it to Biodentine. The physicochemical properties, biocompatibility, antimicrobial capacity and bioactive potential of these materials will be evaluated. The setting time, radiopacity and solubility tests will be based on ISO 6876. The pH measurement will be carried out by digital pHmeter. MTT and Neutral Red tests will be performed to define the dilution of the eluted material. Subsequently, new MTT assay, and alkaline phosphatase assay will be performed to assess cell viability and bioactivity after contact with the material elute for 3 and 7 days. Alizarin Red assay will be performed to identify calcium deposits in cell culture after 21 days of contact with the cement. The antimicrobial activity test will be performed by direct contact method of bacteria in planktonic form. The data obtained for each evaluation will be submitted to appropriate statistical tests with significance level of 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUEIROZ, MARCELA B.; TORRES, FERNANDA F. E.; RODRIGUES, ELISANDRA M.; VIOLA, KENNIA S.; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE M.; SOUZA, MARINA T.; ZANOTTO, EDGAR D.; GUERREIRO-TANOMARU, JULIANE M.; TANOMARU-FILHO, MARIO. Development and evaluation of reparative tricalciumsilicate-ZrO2-Biosilicatecomposites. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 109, n. 4, p. 468-476, APR 2021. (15/03437-6, 16/11036-4, 16/00321-0, 17/19049-0)

QUEIROZ, MARCELA BORSATTO; ESTEVES TORRES, FERNANDA FERRARI; RODRIGUES, ELISANDRA MARCIA; VIOLA, KENNIA SCAPIN; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE MORAIMA; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers. Dental Materials, v. 37, n. 2, p. 311-320, FEB 2021. (16/00321-0, 17/19049-0, 16/11036-4, 17/14305-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

QUEIROZ, Marcela Borsatto. Physicochemical and biological properties of tricalcium silicate-based reparative materials with alternative radiopacifiers and Biosilicate. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.