Calcium silicate cements are developed as repair materials. Biosilicate is a bioactive glass-ceramic fully crystallized. This project is divided into two subprojects. 1- To develop and evaluate a new material based on tricalcium silicate associated with the radiopacifier calcium tungstate or niobium oxide and compare it to the MTA HP. 2 - To develop and evaluate a new material based on tricalcium silicate associated with the radiopacifier zirconium oxide and 10 or 20% of Biosilicate, and compare it to Biodentine. The physicochemical properties, biocompatibility, antimicrobial capacity and bioactive potential of these materials will be evaluated. The setting time, radiopacity and solubility tests will be based on ISO 6876. The pH measurement will be carried out by digital pHmeter. MTT and Neutral Red tests will be performed to define the dilution of the eluted material. Subsequently, new MTT assay, and alkaline phosphatase assay will be performed to assess cell viability and bioactivity after contact with the material elute for 3 and 7 days. Alizarin Red assay will be performed to identify calcium deposits in cell culture after 21 days of contact with the cement. The antimicrobial activity test will be performed by direct contact method of bacteria in planktonic form. The data obtained for each evaluation will be submitted to appropriate statistical tests with significance level of 5%.
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