Role of insulin during acute inflammation induced by Aeromonas hydrophila in Nile Tilapia Oreochromis niloticus

• Grant number: 16/08891-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2016
• Effective date (End): June 30, 2017
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Julieta Rodini Engracia de Moraes
• Grantee: Elvis Pulici
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

There is clear evidence demonstrating the involvement of the endocrine system in the inflammatory process. While it is of great importance, no shortage of literature on the interaction of the inflammatory process with pancreatic hormones in fish. However, the objective of this study is to evaluate the effect of insulin and glucagon in acute inflammation induced by Aeromonas hydrophila inactivated in Oreochromis niloticus (Nile tilapia). For this, 960 tilapia of Nile (± 200g) will be distributed in 8 groups (3 repetitions): T1: (control group) - treated with sterile saline 0.65%; T2: treated with 30 mg / kg metyrapone; T3: treated with 30 mg / kg metyrapone + 0.75 mg / kg glucagon; T4: treated with 30 mg / kg metyrapone + 0.8 mg / kg of cortisol; T5: metyrapone treated with 30 mg / kg + 0.75 mg / kg glucagon + 0.8 mg / kg of cortisol, T6: treated with 2 IU of insulin; T7: treated with 40 mg / kg of alloxan and T8: treated with 40 mg / kg alloxan + 2 IU of insulin. All treatments will be made up of a group challenged with A. hydrophila inactivated and will have a control group injected with saline. The animals undergo euthanasia at the predetermined time of 30 min, 1, 3, 6, and 24 hours after challenge with A. hydrophila inactivated swim bladder for collection of biological material. The animals will be assessed for their clinical behavior, inflammatory cell component, hematologic variables, serum biochemistry, cortisolemia and glucose. (AU)