Qualitative and quantitative analysis of the anatomical structure of genetically modified plants for the lignin content

• Grant number: 16/14309-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2016
• End date: May 31, 2017
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Paula Macedo Nobile
• Grantee: Jasmim Felipe de Oliveira
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated research grant: 13/19214-0 - Sugarcane gene functional analysis in transgenic rice and lignin modified sugarcane in order to cellulosic ethanol production, AP.JP

Abstract

Sugarcane represents a high potential as crop to cellulosic ethanol yield. However, lignin, which constitutes the secondary cell wall, is a recalcitrant biopolymer to saccharification. The metabolic engineer allows manipulate lignin biosynthesis pathway such a way that improve the cellulosic ethanol yield. Two genes related to the lignin pathway, SHINE and F5H, were integrated into rice and sugarcane genomes, respectively, to evaluated the transgenes overexpression impact on the genetically transformed plants. The present proposal aims to evaluate the transgenic plants rice-OX-SHN and sugarcane-OX-F5H for the lignin deposition in cellular levels as well as its dosage in plant tissues. For this purpose, it will be performed hystochemical tests using the Maule and floruglucinol dyes and, it will be developed methods to the cellular parameters quantification, as the tracheary elements and fibers cell wall thickness measures, lumens and vessels diameters, in order to evaluate the SHINE and F5H overexpression in the structural cell formation. The lignin dosage will be performed by Klason method. The results obtained in the present work will contribute to evaluate genetic transformation plants that might be less recalcitrant to the saccharification process for cellulosic ethanol production. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOER MARTINS, ALEXANDRE PALMA; BRITO, MICHAEL DOS SANTOS; MAYER, JULIANA LISCHKA S.; PORTILLA LLERENA, JUAN PABLO; OLIVEIRA, JASMIM FELIPE; TAKAHASHI, NATALIA CONGALVES; CARLIN, SAMIRA DOMINGUES; ALINE FLORES BORGES, DENISELE NEUZA; ANDRADE, LARISSA MARA; PEIXOTO-JUNIOR, RAFAEL FAVERO; et al. Ectopic expression of sugarcane SHINE changes cell wall and improves biomass in rice. BIOMASS & BIOENERGY, v. 119, p. 322-334, DEC 2018. (14/23017-9, 14/08468-4, 16/14309-1, 13/19214-0, 14/09422-8)