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Identification of proteins markers of endothelial shear stress intensity

Grant number: 16/02406-2
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 15, 2016
Effective date (End): October 14, 2017
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Alexandre da Costa Pereira
Grantee:Gabriela Venturini da Silva
Supervisor abroad: Cristine E. Seidman
Home Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Research place: Harvard University, Boston, United States  
Associated to the scholarship:13/13526-0 - Identification of protein markers changed by shear stress, BP.DR


Endothelial cells have important role in cardiovascular system, mainly for protection and regulation of arteries and veins. These cells are constantly exposed to many factors both as chemical, such as cytokine, chemokine and other signaling molecules; and as mechanicals such as shear stress (SS) and stretch. One of these mechanical forces, the SS, is caused by the direct contact of these cells with blood flow. Shear stress is the parallel force of friction exerted by blood flow on the endothelial cells surface of the arterial wall. The modulation of shear stress intensity is able to change cells in many ways such as protein profile, secretory profile and intracellular/extracellular signaling. High or laminar shear stress is frequently associated with protection of vessels by activation of anti-atherogenic, anti-thrombotic and anti-inflammatory proteins; whereas low or oscillatory shear stress may contribute to endothelial dysfunction, increasing the adhesion molecules and endothelial permeability, thereby contributing to cardiovascular disease. Despite the relationship between SS and atherosclerotic plaques have been explored using different techniques, there are few studies using proteomics and cellular model of SS aiming to identify modulated proteins with potential use for developing of markers of atherosclerosis.Here, we intend to identify differentially regulated proteins in endothelial cells submitted to laminar and low shear stress using proteomic technics, and associate some target proteins to atherosclerosis development.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VENTURINI, GABRIELA; MALAGRINO, PAMELLA ARAUJO; PADILHA, KALLYANDRA; TANAKA, LEONARDO YUJI; LAURINDO, FRANCISCO RAFAEL; DARIOLLI, RAFAEL; CARVALHO, VALDEMIR MELECHCO; MORAIS CARDOZO, KARINA HELENA; KRIEGER, JOSE EDUARDO; PEREIRA, ALEXANDRE DA COSTA. Integrated proteomics and metabolomics analysis reveals differential lipid metabolism in human umbilical vein endothelial cells under high and low shear stress. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v. 317, n. 2, p. C326-C338, AUG 2019. Web of Science Citations: 0.

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