|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||August 01, 2016|
|Effective date (End):||July 31, 2018|
|Field of knowledge:||Health Sciences - Dentistry - Endodontics|
|Principal researcher:||Larissa Moreira Spinola de Castro Raucci|
|Grantee:||Nadyne Saab Messias|
|Home Institution:||Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil|
Calcium aluminate cement (CAC) was developed at Federal University of São Carlos for endodontic purpose as an alternative to mineral trioxide aggregate cement (MTA). Initial studies showed CAC exhibits better mechanical properties than MTA and promising biological results both in osteoblastic cells in vitro and in bone defects when applied in vivo. However, the effects of CAC on odontoblasts are not known. Considering the potential application of this new biomaterial in pulp regenerative therapies, the aim of this study is to evaluate different aspects of the progression of odontoblast-like cell cultures of MDPC-23 cell line exposed to different preparations of calcium aluminate cement, using MTA as control. The following parameters will be assessed: 1) cell morphology at days 1 and 3; 2) Cell viability at days 1, 3 and 7; 3) alkaline phosphatase activity at days 7 and 10; 4) matrix mineralization at day 14.