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Impact of dentin treatment with Er:YAG laser or sodium ascorbate on cementation of glass fiber posts

Grant number: 16/12960-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2016
Effective date (End): August 31, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Aline Evangelista de Souza Gabriel
Grantee:Laís Lima Pelozo
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The treatment of intraradicular dentin with antioxidant agents and laser irradiation aim to make the dental surface more receptive to adhesive procedures. The purpose of this study is to evaluate the effect of Er:YAG laser irradiation or sodium ascorbate solution on intraradicular dentin on glass fiber post cementation. Forty eight bovine incisor roots will be standardized at 15 mm of length and will be submitted to biomechanical preparation up to #70 endodontic file. Root canals will be filled with Sealer 26 using the lateral condensation technique. After the setting time, the removal procedure will be done with a #5 largo drill. The roots will be divided into 4 groups according to intraradicular dentin treatment (n=12): G1- irrigation with distilled water (control); G2 - 10% sodium ascorbate solution (10 min); G3 - Er:YAG laser (60 mJ/4Hz and 20 s) and G4 - Er:YAG laser with 10% sodium ascorbate solution. Glass fiber posts will be cemented into root canals with Relyx U200. The roots will be sectioned into slices and the following variables will be analyzed: 1° slice of each third - bond strength of the material to dentin (push out test) and failure pattern in confocal laser scanning microscope (CLSM); 2° slice of each third - Knoop microhardness (KHN) of the dentin adjacent to the canal, and the cement penetration on dentin under CLSM. Data will be subjected to the appropriate statistical analysis for each test (p<0.05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PELOZO, LAIS LIMA; SILVA-NETO, REINALDO DIAS; MILORI CORONA, SILMARA APARECIDA; PALMA-DIBB, REGINA GUENKA; SOUZA-GABRIEL, ALINE EVANGELISTA. Dentin pretreatment with Er:YAG laser and sodium ascorbate to improve the bond strength of glass fiber post. Lasers in Medical Science, v. 34, n. 1, p. 47-54, . (16/12960-7)

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