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Global proteome profile of periodontal ligament

Grant number: 16/02942-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2016
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Kamila Rosamilia Kantovitz
Grantee:Priscila Alves Giovani
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated scholarship(s):19/13242-9 - Comparative proteomic analysis of dental cementum from deciduous and permanent teeth, BE.EP.DR


It has been suggested that there are histological and functional distinctions between the periodontal ligament (PDL) of deciduous (DecPDL) and permanent (PermPDL) teeth. Thus, we hypothesized that DecPDL and PermPDL display differences in the constitutive expression of genes/proteins involved with PDL homeostasis. Primary PDL cell cultures will be obtained for DecPDL (n=3) and PermPDL (n=3) to allow us to perform label-free quantitative secretome analysis. Next, total RNA and protein extracts will be obtained from fresh PDL tissues of deciduous (n=6) and permanent (n=6) teeth, and Western blotting and qPCR analysis were used to validate our in vitro findings. In order to systematically acess potential morphological differences between DecPDL and PermPDL, we will use polarizing and light microscopy analysis in histological sections obtained from minipigs. The fold-change calculation will be based on the ratio of the mean of the normalized counts of PermPDL and DecPDL [mean PermPDL / mean DecPDL] and the beta-binomial model for analysis of spectrum counts to determine the differentially and exclusively proteins expressed. Data from protein (Western Blotting) and gene (qPCR) expression were analyzed by Student´s t test (±d0,05), while the histological results will be presented descriptively.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE ANDRADE, PEDRO AVELINO MAIA; GIOVANI, PRISCILA ALVES; ARAUJO, DARLLE SANTOS; DE SOUZA, ADIJAILTON JOSE; PEDRONI-PEREIRA, ALINE; KANTOVITZ, KAMILA ROSAMILIA; ANDREOTE, FERNANDO DINI; CASTELO, PAULA MIDORI; NOCITI-JR, FRANCISCO HUMBERTO. Shifts in the bacterial community of saliva give insights on the relationship between obesity and oral microbiota in adolescents. Archives of Microbiology, FEB 2020. Web of Science Citations: 0.
GIOVANI, PRISCILA A.; SALMON, CRISTIANE R.; MARTINS, LUCIAN; LEME, ADRIANA F. P.; PUPPIN-RONTANI, REGINA M.; MOFATTO, LUCIANA S.; NOCITI JR, FRANCISCO H.; KANTOVITZ, KAMILA R. Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth. Journal of Periodontology, v. 90, n. 7, p. 775-787, JUL 2019. Web of Science Citations: 0.

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