Molecular markers related to energy metabolism and its association with substrate oxidation and resting metabolic rate in women with morbid obesity before and after bariatric surgery

• Grant number: 15/06624-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): September 01, 2016
• Effective date (End): February 28, 2019
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Carla Barbosa Nonino
• Grantee: Bruno Affonso Parenti de Oliveira
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The mechanisms of genetic contribution to development of obesity need to be established in interventions that aim to weight loss, as bariatric surgery. Therefore, the analysis of gene expression by microarray related energy expenditure and subsequent association with the resting metabolic rate and energy metabolism may elucidate the understanding of genetic factors that contribute to obesity. The aim of this study is analyze molecular markers related to energy metabolism and substrate oxidation associated with measures and resting metabolic rate in women with obesity III grade before and after bariatric surgery. The sample consisted of 20 women of mixed population, aged 18 to 60 years, divided into two groups: group 1 (G1) 10 women with obesity III grade candidates for bariatric surgery followed at the Bariatric Surgery Clinic of HCFMRP- USP and group 2 (G2) control group of 10 normal. Nutritional assessment will be made by Body Mass Index (BMI), body composition by bioelectrical impedance method, Resting Metabolic Rate (RMR), glucose oxidation and lipid by calorimetry indirect. To assess genetic profile, will be collected peripheral blood and use microarray methodology for BeadChip and then validate by quantitative PCR method in Real Time. After six month, participants underwent bariatric surgery will be re-evaluated and compared with control group, pre and post surgery. Results will be analyzed by SPSS software version 17.0 and the level of significance will be established at p <0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PARENTI DE OLIVEIRA, BRUNO AFFONSO; NICOLETTI, CAROLINA FERREIRA; DE OLIVEIRA, CRISTIANA CORTES; DE SOUZA PINHEL, MARCELA AUGUSTA; GOMES QUINHONEIRO, DRIELE CRISTINA; NORONHA, NATALIA YUMI; MARCHINI, JULIO SERGIO; NONINO, CARLA BARBOSA. A new resting metabolic rate equation for women with class III obesity. NUTRITION, v. 49, p. 1-6, MAY 2018. (15/06624-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Bruno Affonso Parenti de. Molecular markers related to energy metabolism and its association with substrate oxidation and resting metabolic rate in women with grade III obesity before and after bariatric surgery. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.