Antimicrobial activity of metabolites produced by Streptococcus mutans on Candida albicans in in vivo study models

• Grant number: 16/03395-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2016
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Juliana Campos Junqueira
• Grantee: Jéssica Diane dos Santos
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

"In vitro" studies have shown that Streptococcus mutans can produce metabolites capable of inhibiting Candida albicans, becomming interesting the identification and development of new substances for the treatment of oral candidiasis. The aim of this study is to purify and identify the metabolites produced by S. mutans and evaluate their effects on the pathogenicity of C. albicans and on the immune response in "in vivo" models study. The supernatant metabolites of S. mutans culture will be extracted with ethyl acetate and then fractionated and identified by High-performance Liquid Chromatography - Diode Array Detection (HPLC-DAD). Hereafter, the effects of the metabolites will be assessed on experimental candidiasis in Galleria mellonella and in immunosuppressed mice. In experimental infection model with G. mellonella, the effects of the metabolites will be analyzed by survival curve test, quantitation of CFU/ml of C. albicans in hemolymph, phagocytosis and determining of hemocyte density of G. mellonella larvae. The S. mutans metabolite with greater antimicrobial activity against C. albicans in G. mellonella will be selected for the study of oral candidiasis in mice. In this experimental model, the development of candidosis will be evaluated by recovery tests and determination of CFU/ml of C. albicans from the mice's oral cavity, macroscopic and microscopic examination of the tongue dorsum and quantification of cytokines from the serum of the animals. The results of the survival experiments in G. mellonella will be analyzed by log-rank test (Mantel-Cox). The data obtained in the other tests will be submitted to an exploratory analysis for the selection of the most appropriate statistical test for each experiment. In all tests will be used GraphPad Prism 5.0 program, considering a significance level of 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, JESSICA DIANE; DE OLIVEIRA FUGISAKI, LUCIANA RUANO; MEDINA, REBECA PREVIATE; SCORZONI, LILIANA; ALVES, MARIANA DE SA; DE BARROS, PATRICIA PIMENTEL; RIBEIRO, FELIPE CAMARGO; FUCHS, BETH BURGWYN; MYLONAKIS, ELEFTHERIOS; SIQUEIRA SILVA, DULCE HELENA; et al. Streptococcus mutansSecreted Products InhibitCandida albicansInduced Oral Candidiasis. FRONTIERS IN MICROBIOLOGY, v. 11, JUL 15 2020. (16/03395-4, 16/05226-5)

ALVES, MARIANA DE SA; DE OLIVEIRA FUGISAKI, LUCIANA RUANO; DOS SANTOS, JESSICA DIANE; SCORZONI, LILIANA; MEDINA, REBECA PREVIATE; SIQUEIRA SILVA, DULCE HELENA; JUNQUEIRA, JULIANA CAMPOS. Antifungal effects of Streptococcus mutans extract on Candida strains susceptible and resistant to fluconazole: An in vivo study. Medical Mycology, v. 59, n. 7, p. 744-747, JUL 2021. (16/03395-4, 16/05226-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Jéssica Diane dos. Antifungal activity of crude extract and fractions of Streptococcus mutans on Candida albicans in models of in vivo study. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.