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Functional analysis of candidate effector proteins during Sporisorium scitamineum x sugarcane interaction

Grant number: 16/16376-8
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2016
Effective date (End): January 31, 2019
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal Investigator:Claudia Barros Monteiro Vitorello
Grantee:Natália de Sousa Teixeira e Silva
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated scholarship(s):17/25477-5 - Identification of candidate interactors and global response pathways activated by Sporisorium scitamineum effectors, BE.EP.DR


Sugarcane smut is a worldwide distributed disease important to agribusiness, since it can affect cane yield drastically. The disease is caused by the Basidiomycete fungus Sporisorium scitamineum, a biotrophic species that colonizes particularly sugarcane. Cane-smut interaction has been studied by our research group for the past few years in their various aspects, considering both attack of the pathogen and plant defenses. The aim of this project is to study candidate effector proteins of this pathosystem. Effectors are essential to modulate host metabolism to allow pathogen colonization. The identification of such proteins may assist in the recognition of resistance genes relevant to genetic breeding programs. Based on the complete genome sequence of S. scitamineum, candidate genes will be selected in silico (TANIGUTI et al., 2015). Selection strategies will be based on the translated amino acid sequence features, differential expression level of the genes in planta, previously obtained in RNAseq assay (SCHAKER et al., submitted), and genetic diversity studies (BENEVENUTO et al., submitted). Candidate effectors will be analyzed regarding their subcellular location in plant cells using agroinfiltration for transient expression in Nicotiana benthamiana plants. Subsequently, a co-immunoprecipitation assay will be performed to capture protein complexes and evaluate their direct or indirect interaction. The selected candidate genes will have their expression level validated by RT-qPCR. These results will enable future researches considering virulence level of different isolates and also help decision making in plant breeding programs.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PETERS, L. P.; TEIXEIRA-SILVA, N. S.; BINI, A. P.; SILVA, M. M. L.; MORAES, N.; CRESTANA, G. S.; CRESTE, S.; AZEVEDO, R. A.; CARVALHO, G.; MONTEIRO-VITORELLO, C. B.. Differential responses of genes and enzymes associated with ROS protective responses in the sugarcane smut fungus. FUNGAL BIOLOGY, v. 124, n. 12, p. 1039-1051, . (17/13268-2, 18/07615-4, 16/16376-8, 13/15014-7, 16/17545-8)
TEIXEIRA-SILVA, NATALIA SOUSA; CARVALHO SCHAKER, PATRICIA DAYANE; SILVA RODY, HUGO VIANNA; MAIA, THIAGO; GARNER, CHRISTOPHER M.; GASSMANN, WALTER; MONTEIRO-VITORELLO, CLAUDIA BARROS. Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors. JOURNAL OF FUNGI, v. 6, n. 4, . (17/13268-2, 18/04555-0, 16/04429-0, 16/16376-8, 17/25477-5, 16/17545-8, 13/25599-2)
BENEVENUTO, JULIANA; TEIXEIRA-SILVA, NATALIA S.; KURAMAE, EIKO E.; CROLL, DANIEL; MONTEIRO-VITORELLO, CLAUDIA B.. Comparative Genomics of Smut Pathogens: Insights From Orphans and Positively Selected Genes Into Host Specialization. FRONTIERS IN MICROBIOLOGY, v. 9, . (17/13268-2, 16/03768-5, 16/16376-8, 16/17545-8, 14/21802-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Natália de Sousa Teixeira e. Functional analysis of candidate effector proteins during Sporisorium scitamineum x sugarcane interaction. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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