Lactic acid bacteria in Brazilian artisanal cheeses: application of Evolutionary Engineering for culture improvement aiming at accelerating cheese ripening

• Grant number: 15/25641-4
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: November 01, 2016
• End date: August 19, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Products of Animal Origin Inspection
• Principal Investigator: Anderson de Souza Sant'Ana
• Grantee: Larissa Pereira Margalho
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 17/03899-5 - Acid lactic bacteria and yeasts in Brazilian artisanal cheeses: characterization and selection by evolutionary engineering to improve cheese processing, BE.EP.DD

Abstract

Currently, there are several types of artisanal cheeses in Brazil, which have peculiar characteristics, according to their origin, heritage, culture and lifestyle of the producing communities. Among these, cheese produced in the regions of Araxá, Serra da Canastra, Alto Paranaíba and Serro; Manteiga and Coalho produced in the Northeast; Colonial and Serrano, produced in the South, stand out. The particular microflora of these cheeses, associated with the region of origin and manufacturing technology, consists mainly of lactic acid bacteria and yeasts present in milk and/or from the processing environment. This microbiota is responsible for biochemical reactions during the ripening process, which impart in aroma and flavors compounds. However, during the process of pasteurization for the elimination of pathogens, there is a sudden reduction of this endogenous microbiota, which is known to influence on sensory property of cheeses. A ripening period greater than 60 days suggested by the legislation to reduce pathogens in cheeses made from raw milk also mischaracterizes the typical Brazilian artisanal cheeses. Considering the appeal of consumers for healthier, natural, safe and local products, this project aims to the isolate lactic acid bacteria and yeasts from Brazilian artisanal cheeses, to characterize these isolates through phenotypical and genotypícal tests and through their technological properties and probiotic potential. Finally, the project aims to develop a more robust culture(s) through the Evolutionary Engineering approach aiming to accelerate the ripening process of Brazilian artisanal cheeses, while ensuring quality (sensory properties) and microbiological safety. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARGALHO, LARISSA P.; KAMIMURA, BRUNA A.; PIMENTEL, TATIANA C.; BALTHAZAR, CELSO F.; ARAUJO, JASMIM V. A.; SILVA, RAMON; CONTE-JUNIOR, CARLOS A.; RAICES, RENATA S. L.; CRUZ, ADRIANO G.; SANT'ANA, ANDERSON S.. A large survey of the fatty acid profile and gross composition of Brazilian artisanal cheeses. Journal of Food Composition and Analysis, v. 101, AUG 2021. (18/24540-8, 16/12406-0, 15/25641-4, 13/20456-9)

CAMPAGNOLLO, FERNANDA B.; MARGALHO, LARISSA P.; KAMIMURA, BRUNA A.; FELICIANO, MARCELO D.; FREIRE, LUISA; LOPES, LETICIA S.; ALVARENGA, VERONICA O.; CADAVEZ, VASCO A. P.; GONZALES-BARRON, URSULA; SCHAFFNER, DONALD W.; et al. Selection of indigenous lactic acid bacteria presenting anti-listerial activity, and their role in reducing the maturation period and assuring the safety of traditional Brazilian cheeses. FOOD MICROBIOLOGY, v. 73, p. 288-297, AUG 2018. (14/14891-7, 13/20456-9, 16/12406-0, 15/25641-4, 16/21041-5, 16/09346-5)

RAMOS, GUSTAVO LUIS P. A.; NASCIMENTO, JANAINA S.; MARGALHO, LARISSA P.; CRUZ, ADRIANO G.; SANT'ANA, ANDERSON S.. Quantitative risk assessment for type A staphylococcal enterotoxin poisoning due to consumption of Minas Frescal cheese in Brazil. INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, v. N/A, p. 12-pg., 2022-12-09. (15/25641-4, 20/14631-6)

MARGALHO, LARISSA P.; VAN SCHALKWIJK, SASKIA; BACHMANN, HERWIG; SANT'ANA, ANDERSON S.. Enterococcus spp. in Brazilian artisanal cheeses: Occurrence and assessment of phenotypic and safety properties of a large set of strains through the use of high throughput tools combined with multivariate statistics. FOOD CONTROL, v. 118, DEC 2020. (15/25641-4, 17/03899-5)

MARGALHO, LARISSA P.; JORGE, GENESY P.; NOLETO, DEISE A. P.; SILVA, CHRISTIAN E.; ABREU, JULIA S.; PIRAN, MARCOS V. F.; BROCCHI, MARCELO; SANT'ANA, ANDERSON S.. Biopreservation and probiotic potential of a large set of lactic acid bacteria isolated from Brazilian artisanal cheeses: From screening to in product approach. MICROBIOLOGICAL RESEARCH, v. 242, JAN 2021. (17/03899-5, 15/25641-4)

DE ANDRADE, BARBARA M.; MARGALHO, LARISSA P.; BATISTA, DIEGO B.; LUCENA, IZYLLA O.; KAMIMURA, BRUNA A.; BALTHAZAR, CELSO F.; BREXO, RAMON PERES; PIA, ARTHUR K. R.; COSTA, RAMON A. S.; CRUZ, ADRIANO G.; et al. Chemometric classification of Brazilian artisanal cheeses from different regions according to major and trace elements by ICP-OES. Journal of Food Composition and Analysis, v. 109, p. 9-pg., 2022-03-20. (13/20456-9, 16/12406-0, 18/24540-8, 15/25641-4)

MARGALHO, LARISSA P.; KAMIMURA, BRUNA A.; BREXO, RAMON P.; ALVARENGA, VERONICA O.; CEBECI, ALP SABRI; JANSSEN, PATRICK W. M.; DIJKSTRA, ANNEREINOU; STARRENBURG, MARJO J. C.; SHEOMBARSING, RESHTRIE S.; CRUZ, ADRIANO G.; et al. High throughput screening of technological and biopreservation traits of a large set of wild lactic acid bacteria from Brazilian artisanal cheeses. FOOD MICROBIOLOGY, v. 100, DEC 2021. (15/25641-4, 17/03899-5)

MOLLER, CLEIDE OLIVEIRA DE ALMEIDA; FREIRE, LUISA; ROSIM, ROICE ELIANA; MARGALHO, LARISSA PEREIRA; BALTHAZAR, CELSO FASURA; FRANCO, LARISSA TUANNY; SANT'ANA, ANDERSON DE SOUZA; CORASSIN, CARLOS HUMBERTO; RATTRAY, FERGAL PATRICK; OLIVEIRA, CARLOS AUGUSTO FERNANDES DE. Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of Aspergillus parasiticus, and Their Ability to Bind Aflatoxin B-1, Ochratoxin A, and Zearalenone in vitro. FRONTIERS IN MICROBIOLOGY, v. 12, APR 22 2021. (17/20081-6, 17/03899-5, 15/25641-4, 16/21041-5, 18/24540-8)

DELORME, MARIANA M.; GUIMARAES, JONAS T.; COUTINHO, NATHALIA M.; BALTHAZAR, CELSO F.; ROCHA, RAMON S.; SILVA, RAMON; MARGALHO, LARISSA P.; PIMENTEL, TATIANA C.; SILVA, MARCIA C.; FREITAS, MONICA Q.; et al. Ultraviolet radiation: An interesting technology to preserve quality and safety of milk and dairy foods. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 102, p. 146-154, AUG 2020. (15/25641-4, 17/03899-5)

LAROSA, CRISTIANE P.; BALTHAZAR, CELSO F.; GUIMARAES, JONAS T.; MARGALHO, LARISSA P.; LEMOS, FELIPE S.; OLIVEIRA, FELIPE L.; ABUD, YURI K. D.; SANT'ANNA, CELSO; DUARTE, MARIA CARMELA K. H.; GRANATO, DANIEL; et al. Can sucrose-substitutes increase the antagonistic activity against foodborne pathogens, and improve the technological and functional properties of sheep milk kefir?. Food Chemistry, v. 351, JUL 30 2021. (15/25641-4, 17/03899-5)

CAMPAGNOLLO, FERNANDA B.; FURTADO, MARIANNA M.; SILVA, BEATRIZ S.; MARGALHO, LARISSA P.; CARMINATI, JOYCE A.; SANT'ANA, ANDERSON S.; NASCIMENTO, MARISTELA S.. A quantitative risk assessment model for salmonellosis due to milk chocolate consumption in Brazil. FOOD CONTROL, v. 107, JAN 2020. (15/25641-4, 14/14891-7, 09/11406-2)

MARGALHO, LARISSA P.; FELICIANO, MARCELO D'ELIA; SILVA, CHRISTIAN E.; ABREU, JULIA S.; PIRAN, MARCOS VINICIUS FIORENTINI; SANT'ANA, ANDERSON S.. Brazilian artisanal cheeses are rich and diverse sources of nonstarter lactic acid bacteria regarding technological, biopreservative, and safety properties-Insights through multivariate analysis. JOURNAL OF DAIRY SCIENCE, v. 103, n. 9, p. 7908-7926, SEP 2020. (17/03899-5, 15/25641-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARGALHO, Larissa Pereira. Bioprospecção do potencial tecnológico, probiótico e avaliação da segurança de bactérias láticas endógenas de queijos artesanais brasileiros. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.