Study of the role of angiotensin II on in vitro differentiation of human apical papilla cells

• Grant number: 16/16473-3
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): November 01, 2016
• Effective date (End): August 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Carla Renata Sipert
• Grantee: Laís Nicolay Pizzatto
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach, AP.TEM

Abstract

Pulp revascularization has resulted in more favorable prognosis compared to apicification at the treatment of immature necrotic teeth. Remaining cells from apical papilla have been suggested as the key for the treatment success. However, the molecular mechanisms involved with the modulation of these cells under unfavorable environment such as inflammation remains unclear. Among the mediators involved in this process, angiotensin II (AngII) might be suggested as an important modulator of cellular function. The aim of this study is to elucidate the role of AngII at the osteo/odontogenic differentiation of human apical papilla cells (CPA) in vitro. CPA cultures will be established from third molars with incomplete root formation and characterized. The cells will be subjected to stimulum with increasing concentrations of lipopolysaccharide (LPS) for cellular viability assay. Then, CPA will be challenged with LPS at proliferation (MP) or differentiation medium (MD) for detection of Ang II production and the receptor AT1. Its role at cellular differentiation will be investigated by means of pharmacological blockade. Data will be analyzed by means of GraphPad Prism 5.0 using One-way analysis of variance (ANOVA) followed by Tukey´s post-test. Significant values will be set at p < 0.05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOSIO MENESES, CLAUDIA CAROLINE; PIZZATTO, LAIS NICOLAY; FARES ANDRADE, FERNANDA FACHINI; SIPERT, CARLA RENATA. Prostaglandin E-2 Affects Interleukin 6 and Monocyte Chemoattractant Protein 1/CCL2 Production by Cultured Stem Cells of Apical Papilla. JOURNAL OF ENDODONTICS, v. 46, n. 3, p. 413-418, MAR 2020. (16/13944-5, 16/16473-3, 17/01737-8)

PIZZATTO, LAIS NICOLAY; MENESES, CLAUDIA C. B.; DINIZ, ELISA A.; DIONISIO, THIAGO J.; SANTOS, CARLOS FERREIRA; SIPERT, CARLA R.. Angiotensin II Regulates Proliferation and Function of Stem Cells of Apical Papilla. JOURNAL OF ENDODONTICS, v. 46, n. 6, p. 810-817, JUN 2020. (17/01737-8, 16/16473-3, 16/13944-5, 15/03965-2)

MENESES, CLAUDIA CAROLINE BOSIO; PIZZATTO, LAIS N.; SIPERT, CARLA R.; DIOGENES, ANIBAL. Endocannabinoids Regulate Stem Cells of the Apical Papilla via a Cannabinoid Receptor and TRPV1-Independent Mechanism. JOURNAL OF ENDODONTICS, v. 47, n. 10, p. 1617-1624, OCT 2021. (16/16473-3, 16/13944-5, 18/21869-9, 17/01737-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PIZZATTO, Laís Nicolay. Angiotensin II in the modulation of apical human papilla cells in vitro. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.