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Development of nanostructured lipid carriers as carrier system of curcumin and in vitro biological evaluation in bladder cancer cells

Grant number: 16/16921-6
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2016
Effective date (End): July 31, 2017
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Priscyla Daniely Marcato Gaspari
Grantee:Jacqueline Campos Silva
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Considered the second most common malignancy of the urinary tract, bladder cancer (BC) causes great concern due to the alarming estimates that grow every year. Among the therapies used in the BC, stand out BCG immunotherapy and chemotherapy. However, their efficiency is limited due to some factors such as, for example, the unwanted side effects and the high rate of recurrence after treatment. Thus, studies are being conducted on the big natural reserve of organic compounds as a strategy to find chemotherapeutic agents that are more selective, effective and with less toxicity. In this way, the oleoresins of several species of Copaifera and phenolic compounds as curcumin have been the subject of research due to its numerous pharmacological and therapeutic properties, as the antitumor effect. Besides the choice of a most efficient chemotherapy, some challenges in intravesical therapy of BC must be overcome, for example, low permeation of molecules in the urothelium and low residence time of drug in the bladder, which require multiple administrations, causing discomfort to the patient and risk of infections. In order to overcome these barriers, sustained drug release systems have been used. These systems achieve higher internalization of the drug in tumor cells, prolonged release, lower toxicity, greater residence time at the site of action and fewer administrations. Among the nanoparticles, nanostructured lipid carriers (NLC) are highlighted due to its high stability, easy production on a large scale and high encapsulation efficiency of lipophilic molecules. Thus, this project aims at the development and characterization of NLC prepared with Copaifera duckei oleoresin (Cd-O) as carrier system of curcumin (CM), for possible future use in intravesical therapy of BC. The particles will be prepared by the method of emulsion and sonication and will be characterized according to diameter and zeta potential by spectroscopy photon correlation, the morphology by atomic force microscopy, and the crystallinity by differential scanning calorimetry. The encapsulation efficiency of CM and its release profile will also be evaluated. The effectiveness of these nanostructured systems will be analyzed by cytotoxicity assays in bladder cancer cells (RT4) in 2D and 3D culture models.

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Jacqueline Campos. Development of nanostructured lipid carriers as carrier system of Curcuma longa extract and in vitro biological evaluation in bladder cancer cells. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Ribeirão Preto.

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