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Evaluation of microvesicles effect on adherence and replication of human tubular cells in decellularized matrix

Grant number: 16/16613-0
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2016
Effective date (End): November 30, 2018
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Nádia Karina Guimarães de Souza
Grantee:Eliezer Francisco de Santana Junior
Home Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil

Abstract

Chronic kidney disease is a worldwide growing problem. The kidney has the ability for nearly complete regenerate after ischemia/reperfusion or toxic injury. However, in some injuries the kidney develops fibrosis with loss of function. In last years the progression mechanisms for kidney disease and possible interventions have been on focus of studies.Some progression factors are well known, as some growth factors that can lead to regeneration have been described. Hepatocyte growth factor has an important hole as a regulatory factor for regeneration of renal cells.Different cell types had been proposed for treatment of renal fibrosis such as bone marrow mesenchymal stem cells, multipotent cells from the kidney, embryonic stem cells and primary renal cells. Primary renal cells are more numerous and easier to expand than stem cells. Recently, cellular cross talk between mesenchymal stem cells and renal epithelial cells has been recognized. HGF presence in microvesicles produced by mesenchymal stem cells may be a source for regeneration stimulus. The main purpose of this project is to evaluate the effect of microvesicles from umbilical mesenchymal stem cells on the adherence and proliferation of primary renal cell growing in a decellularizated porcine matrix.