Metaproteomics analyses of root canal content of teeth with endodontic failure

• Grant number: 16/19743-1
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: December 27, 2016
• End date: January 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Brenda Paula Figueiredo de Almeida Gomes
• Grantee: Priscila Amanda Francisco
• Supervisor: Walter Luiz Siqueira
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: Western University , Canada
• Associated to the scholarship: 15/19215-2 - Microorganisms and their virulence factors detection in saliva, pulp chamber and root canal of teeth associated with endodontic failure, BP.MS

Abstract

Bacterial persistence or reinfection are the main causes of post-treatment apical periodontitis. Proteome screening of microbial communities is important to provide an insight into the pathogenicity mechanisms and interactions between root canal bacteria and host in the persistence or resurgence of apical periodontitis. Thus, the aims of this study are: a) to characterize the proteome of 20 root canals of teeth with endodontic failure by Mass Spectrometry; and b) to compare the identified proteins with clinical signs and symptoms. Twenty patients with apical periodontitis and need for endodontic retreatment were selected. Samples from the root canal content were collected and will be further processed by Capillary Nano-Flow 2-Dimensional Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry (2D-LC-ESI-MS/MS). All the proposed work involving mass spectrometric analyses will be performed using the in-house LTQ-Velos-ESI-MS/MS instrumentation (Thermo-Scientific, San Jose, CA). The acquired MS/MS spectra generated will be separately searched against specific Sus scrofa protein database (Swiss PROT and TREMBL, Swiss Institute of Bioinformatics, Geneva, Switzerland, http://ca.expasy.org) for all samples using Proteome Discoverer 1.3 software and SEQUEST algorithm. The data will be tabulated and statistically analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCISCO, P. A.; DELBONI, M. G.; LIMA, A. R.; XIAO, Y.; SIQUEIRA, W. L.; GOMES, B. P. F. A.. Proteomic profile of root canal contents in teeth with post-treatment endodontic disease. International Endodontic Journal, v. 52, n. 4, p. 451-460, APR 2019. (17/16516-7, 15/23479-5, 16/19743-1, 15/19215-2)