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Expansion of satellite cell pools in adult skeletal muscle: role of bioenergetics and mitochondrial alterations induced by aerobic exercise

Grant number: 16/18633-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2017
Effective date (End): February 29, 2020
Field of knowledge:Biological Sciences - Physiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Alicia Juliana Kowaltowski
Grantee:Phablo Sávio Abreu Teixeira
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID


Muscle stem cell (satellite cells) function during regeneration is well understood, but its role in response to aerobic exercise remains unclear. Studies have shown increases in the number of satellite cells after physical training. However, if the gain in aerobic fitness, involves the activation and expansion of the satellite cell pool in adult skeletal muscle due to bioenergetics and mitochondrial alterations has not been determined. In this study, we will: a) characterize exercise-induced gains in aerobic fitness and the expansion of satellite cells in different types of fibers (muscle MyHC isoforms); b) verify the expression of bioenergetics and mitochondrial markers and self-renewal of satellite cells at different exercise times; c) based on the targets investigated in (b) mice will be exercised under different conditions (i.e. pharmacological inhibition and gene knockdown in vivo) to evaluate the effects of bioenergetics and mitochondrial alterations induced by aerobic exercise on the activation of satellite cell self-renewal; d) isolate the satellite cells of exercised mice for characterization of the expression of specific bioenergetics and mitochondrial markers, and self-renewal of satellite cells in vitro. The satellite cells activation self-renewal reflects the ability of skeletal muscle to adapt to stressors, such as injuries and muscular disorders (dystrophy, sarcopenia and cachexia). The role of aerobic exercise in increasing the content of satellite cells makes it an important therapeutic strategy against loss of muscle function. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ABREU, PHABLO. Bioenergetics mechanisms regulating muscle stem cell self-renewal commitment and function. BIOMEDICINE & PHARMACOTHERAPY, v. 103, p. 463-472, JUL 2018. Web of Science Citations: 0.
ABREU, PHABLO; DIOGENES MENDES, SAVIO VICTOR; CECCATTO, VANIA MARILANDE; HIRABARA, SANDRO MASSAO. Satellite cell activation induced by aerobic muscle adaptation in response to endurance exercise in humans and rodents. Life Sciences, v. 170, p. 33-40, FEB 1 2017. Web of Science Citations: 11.

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