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Effect of phytotherapeutic drugs incorporated into temporary denture soft lining materials on Candida albicans biofilm

Grant number: 16/08702-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2016
Effective date (End): November 30, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Karin Hermana Neppelenbroek
Grantee:Thaís Yumi Ueda
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The use of phytotherapeutic drugs as an alternative to antifungal/antimicrobial drugs in temporary resilient liners for removable denture has been recently suggested for the treatment of denture stomatitis. A wide variety of plant extracts have showed antifungal activity against Candida albicans, suggesting their potential for the treatment of this pathology. Punica granatum and Equisetum giganteum are phytotherapeutic drugs with bactericidal and fungicidal activities against different species of Staphylococcus, mycobacteria, clinical strains of C. albicans and Escherichia coli. Considering the potential of modification of temporary resilient materials by drugs in the treatment of denture stomatitis and the antifungal activity of P. granatum and E. giganteum, this study aims to evaluate the long-term anifungal action of these phytotherapeutic drugs incorpored into temporary soft materials on the C. albicans biofilm. Different concentrations of the phytotherapeutic drugs P. granatum and E. giganteum incorporated into a tissue conditioner (Softone) and temporary resilient liner (Trusoft) will be tested, and cellular viability was determined qualitatively by spectrophotometric tetrazolium salt XTT reduction assay at 24 h, 48 h, 7 and 14 days of incubation. Quantitative analysis of the biofilm will be assessed by aqueous crystal violet solution. The spectrophotometric measurements will be converted to percentage reduction in candidal growth and the minimum inhibitory concentrations (MICs) determined as the concentrations necessary to inhibit 90% or more of C. albicans viability. The spectrophotometric measurements of XTT colorimetric assay will be converted in percentage of fungal reduction and the minimum inhibitory concentrations (MICs) will be determined as the concentrations necessary to inhibit 90% or more of candidal growth. The absorbance values of the qualitative analysis of the violet crystal will be analyzed by the most appropriate statistical method according to the data distribution. It will be adopted a 5% significance level. (AU)