CHARACTERIZATION OF THE MELANIN-CONCENTRATING HORMONE RECEPTOR 1 (MCHR1) IN THE BRAIN, MAMMARY GLAND, UTERUS AND OVARIES IN WILD TYPE AND MCHR1 KNOCKOUT MICE.

• Grant number: 16/19845-9
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: January 10, 2017
• End date: April 09, 2017
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Jackson Cioni Bittencourt
• Grantee: Jozélia Gomes Pacheco Ferreira
• Supervisor: Antoine Adamantidis
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Bern, Switzerland
• Associated to the scholarship: 15/05990-4 - The melanin-concentrating hormone [MCH] peptidergic system: neurogenesis and lactation, BP.PD

Abstract

Introduction: The melanin-concentrating hormone (MCH) is an important neuropeptide, initially described as an orexigenic factor but associated with a lot of physiological actions, especially in motivated behaviors. Only during lactation it is possible to detect the messenger RNA expression of the prepro-MCH (ppMCH mRNA) and immunoreactivity to MCH in the medial preoptic area (MPOA), a crucial region for maternal behavior expression. Probing the relationship between MCH during the lactation period and peripheral tissues, our group has demonstrated that the dam's mammary gland represents a novel site of MCH receptor 1 (MCHR1) mRNA expression. Therefore, MCH might have a tight relation with peripheral organs related to reproductive system. Aim: Investigate the presence of MCHR1 in the mammary gland, uterus and ovaries of lactating and non-lactating female mice, using the brain as a reference tissue and MCHR1 knockout mice as negative control for all techniques performed. Materials and Methods: The mammary glands, uteri and ovaries of wild type and MCHR1-KO mice in different phases (diestrus, 5th, 12th, 19th days of lactation) will be harvested and processed for alkaline-phosphatase immunohistochemistry.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATTAGELLO, DANIELLA S.; LORENZON, ALINE R.; DINIZ, GIOVANNE B.; MOTTA-TEIXEIRA, LIVIA C.; KLEIN, MARIANNE O.; FERREIRA, JOZELIA G. P.; ARIAS, CARLOS M.; ADAMANTIDIS, ANTOINE; SITA, V, LUCIANE; CIPOLLA-NETO, JOSE; et al. The Rat Mammary Gland as a Novel Site of Expression of Melanin-Concentrating Hormone Receptor 1 mRNA and Its Protein Immunoreactivity. FRONTIERS IN ENDOCRINOLOGY, v. 11, JUL 31 2020. (15/05990-4, 16/02748-0, 17/16293-8, 10/52068-0, 16/04210-8, 17/17998-5, 14/50457-0, 12/04554-8, 14/13052-1, 16/18941-4, 16/19845-9, 16/02224-1)

PACHECO FERREIRA, JOZELIA GOMES; BITTENCOURT, JACKSON CIONI; ADAMANTIDIS, ANTOINE. Melanin-concentrating hormone and sleep. CURRENT OPINION IN NEUROBIOLOGY, v. 44, p. 152-158, JUN 2017. (16/19845-9, 10/52068-0, 04/13849-5, 15/05990-4)

PACHECO FERREIRA, JOZELIA GOMES; GOMES DUARTE, JESSICA CATHARINE; DINIZ, GIOVANNE BARONI; BITTENCOURT, JACKSON CIONI. Litter size determines the number of melanin-concentrating hormone neurons in the medial preoptic area of Sprague Dawley lactating dams. Physiology & Behavior, v. 181, p. 75-79, NOV 1 2017. (16/02224-1, 16/02748-0, 10/52068-0, 15/05990-4, 16/19845-9)

DINIZ, GIOVANNE B.; BATTAGELLO, DANIELLA S.; KLEIN, MARIANNE O.; BONO, BIANCA S. M.; FERREIRA, JOZELIA G. P.; MOTTA-TEIXEIRA, LIVIA C.; DUARTE, JESSICA C. G.; PRESSE, FRANCOISE; NAHON, JEAN-LOUIS; ADAMANTIDIS, ANTOINE; et al. Ciliary melanin-concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex-independent manner. Journal of Neuroscience Research, v. 98, n. 10, JUN 2020. (15/05990-4, 16/02748-0, 17/16293-8, 17/17998-5, 16/18941-4, 16/19845-9, 16/02224-1, 14/22313-3)