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Chlorin as a photosensitizer for photodynamic therapy on Streptococcus mutans

Grant number: 16/09296-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): December 01, 2016
Effective date (End): May 31, 2018
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Juliana Campos Junqueira
Grantee:Maíra Terra Garcia
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


Photodynamic therapy (PDT) has shown to be effective in reducing pathogenic microorganisms from the oral cavity, helping in the treatment of stomatological diseases until then treated in a conventional manner. Different photosensitizers for PDT have been described, including chlorin. However there are no studies in the literature that tested the effects of PDT mediated chlorin against cariogenic bacteria, such as Streptococcus mutans. Thus, the aim of this study is to evaluate the effect of photodynamic therapy (PDT), mediated by the association of the photosensitizer chlorin and laser light of gallium arsenide and aluminum, on planktonic cultures and biofilms formed by S. mutans. For the planktonic cultures study, standardized suspension of S. mutans will be add in 96 well plates. For the biofilm study, enamel fragments will be made from bovine incisor teeth as substrate for the biofilm formation in 24-well plates. The planktonic culture growth and biofilms will be treated according to the experimental groups: photosensitization and laser irradiation (F+G+); only laser irradiation (G-F+); only photosensitizer (F+L-); and a control group without treatment (F-L-). The results will be analyzed by S. mutans CFU/ml counting in agar Brain Heart Infusion (BHI) incubated for 48 h at 37°C under microaerophilic conditions. After the treatments, the biofilms formed on the enamel fragments will be analyzed by Atomic Force Microscopy seeking to detect the effects of PDT on the biofilms surface. Statistical analysis will be performed by Analysis of Variance (ANOVA) and Tukey test. In all experimental tests will be used GraphPad Prism 5.0 software, with 5% of significance level. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TERRA-GARCIA, MAIRA; DE SOUZA, CHEYENNE MARCAL; FERREIRA GONCALVES, NATHALIA MARIA; CORREIA PEREIRA, ANDRE HENRIQUE; DE BARROS, PATRICIA PIMENTEL; BORGES, ALESSANDRA BUHLER; MIYAKAWA, WALTER; STRIXINO, JULIANA FERREIRA; JUNQUEIRA, JULIANA CAMPOS. Antimicrobial effects of photodynamic therapy with Fotoenticine on Streptococcus mutans isolated from dental caries. Photodiagnosis and Photodynamic Therapy, v. 34, . (16/09296-8)
GARCIA, MARIA TERRA; CORREIA PEREIRA, ANDRE HENRIQUE; ALVES FIGUEIREDO-GODOI, LIVIA MARA; CARDOSO JORGE, ANTONIO OLAVO; STRIXINO, JULIANA FERREIRA; JUNQUEIRA, JULIANA CAMPOS. Photodynamic therapy mediated by chlorin-type photosensitizers against Streptococcus mutans biofilms. Photodiagnosis and Photodynamic Therapy, v. 24, p. 256-261, . (16/09296-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GARCIA, Maíra Terra. Clorina com fotossensibilizador para Terapia Fotodinâmica sobre Streptococcus mutans. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.

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