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Effect of whey protein intake on heat shock proteins (HSPs) in obese rats induced by cafeteria diet

Grant number: 16/06630-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): December 01, 2016
Effective date (End): November 30, 2019
Field of knowledge:Health Sciences - Nutrition
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Jaime Amaya Farfan
Grantee:Carolina Soares de Moura
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The heat shock proteins (HSPs) expression can be reduced by obesity. The HSPs classically are part of the natural defense system able to protect and repair cellular damage. The increase and / or restore the expression of HSPs can prevent and / or mitigate damage caused by high-fat diet-induced obesity, promoting an improvement in insulin sensitivity, glucose tolerance and decrease inflammation. HSPs enhancement inhibits the phosphorylation of kinases c-Jun kinase NH2-terminal (JNK) and kB kinase B (IKK²), which are increased in obesity. This approach is suggested as a strategy to improve glucose tolerance in obese. Our researches show that whey protein hydrolysate consumption (WPH) increases HSPs expression in non-obese animals. The high-fat diet have been used to induce obesity in experimental animals. However, this project will use the cafeteria diet model, because is more effective in the induction of obesity features and is a food model very similar to that of humans. The aim of the present study is to investigate the influence of the consumption of WPH on HSPs expression in cafeteria-diet-induced obese animals. So far we have not found any studies to investigate the effect of WPH on HSPs in obese animals, or about the influence of the cafeteria diet. We will use male Wistar rats divided into four groups (n = 8): control (chow, Labina), cafeteria, diet cafeteria added casein and cafeteria added WPH. Will be determined the following blood parameters: glucose, total cholesterol and fractions, triglycerides, total protein, albumin, globulin, AST, ALT, uric acid, insulin, IL-1² interleukins, IL-10, IL-6, C-peptide, GIP, GLP-1 and thiol content. Blood count, glucose tolerance tests, liver histology and glycogen will also be measured. Will evaluate the expression of tissue proteins: HSP70, HSP90, HSP60, HSP25, IKK², JNK, GLUT4, GPx, SOD, catalase, AMPK, Akt, lipase, TLR4, NFk², TNFa, MyD88, MD-2, IL-6, PGC, IDE by western blot. The data will be statistically analyzed using ANOVA and Tukey's test with significance set at p <0.05. The data generated expected to complete a scenario in which one has: on the one hand, components of the diet, which promote chronic illness and, on the other protein components, which act to neutralize the harmful effects of the first. The publication of data on impact journals should contribute to scientific advancement in food and health area. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROQUETTO, ALINE RISSETTI; MOURA, CAROLINA SOARES; SANTOS-JUNIOR, VALFREDO DE ALMEIDA; SAMMARCO OLIVEIRA, PAULO OTAVIO; AIRES MACHADO, KARLA IDELCA; BORGES CASTRO CARVALHO, GESSIKA CRISTINA; RISSO, EDER MULLER; AMAYA-FARFAN, JAIME. Moderate intake of BCAA-rich protein improves glucose homeostasis in high-fat-fed mice. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 80, JUN 2020. Web of Science Citations: 0.
MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; AMAYA-FARFAN, JAIME. Dietary Nutrients and Bioactive Substances Modulate Heat Shock Protein (HSP) Expression: A Review. NUTRIENTS, v. 10, n. 6 JUN 2018. Web of Science Citations: 8.
MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; RISSO, EDER MULLER; AMAYA-FARFAN, JAIME. Modulatory effects of arginine, glutamine and branched-chain amino acids on heat shock proteins, immunity and antioxidant response in exercised rats. FOOD & FUNCTION, v. 8, n. 9, p. 3228-3238, SEP 2017. Web of Science Citations: 10.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.