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Structural and functional stabilization of sericin in a biopolysaccharide hydrogel: bio-origami for skin regeneration

Grant number: 16/16641-3
Support type:Scholarships in Brazil - Master
Effective date (Start): January 01, 2017
Effective date (End): February 28, 2018
Field of knowledge:Health Sciences - Pharmacy
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Vitor Manuel Cardoso Figueiredo Balcão
Grantee:Liliam Katsue Harada Rocha
Home Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil

Abstract

Hydrogels are structures formed by three-dimensional network of crosslinked hydrophilic polymers. The biopolymeric hydrogels are very interesting for use in skin regeneration and treatment of skin wounds, such as bandages, due to many factors, including low toxicity. In preparing hydrogels are used both natural and synthetic polymers, polymerized by different processes. In this research project, provides for the use of biopolysaccharide xanthan and carrageenan gum. The sericin will be incorporate in biohydrogels as active. Sericin is a bioactive globular protein which composes 25-30% of the silk proteins. Due to their unique biochemical and biophysical properties it has been studied for many potential applications. The main objective of this work is to develop and evaluate a biopolymeric hydrogel, integrating sericin with skin regeneration potential. The sericin , extracted from silkworm cocoon (Bombyx mori), will be compared to sericin standard and characterized extensively. Following the characterization of sericin, will be produced and will be optimize biopolymeric hydrogel for functional and structural stabilization of sericin, using full factorial design 32 (2 variables (% (w/w)xanthan gum and % (w/w)carrageenan) at 3 levels ((-1), (0) and (+1)) with the addition of PVA (98% hydrolysed) to 1.25% (w/v) polymerizer, and glycerol to 2.5%(w/v) to induce plasticizing effect, thereby yielding 09 formulations. For the statistically optimized hydrogel, sericin incorporation will be in concentrations of 0, 1, 2, 5, 10, 20 and 50 mgsericina / mLhydrogel. The obtained formulations will be evaluated considering the ability to release the sericin, and characterization tests both physical-chemical and biological. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BALCAO, VICTOR M.; HARADA, LILIAM K.; JORGE, LUDMILLA R.; OLIVEIRA JR, JOSE M.; TUBINO, MATTHIEU; VILA, MARTA M. D. C. Structural and Functional Stabilization of Sericin from Bombyx mori Cocoons in a Biopolysaccharide Film: Bioorigami for Skin Regeneration. Journal of the Brazilian Chemical Society, v. 31, n. 4, p. 833-848, APR 2020. Web of Science Citations: 0.
FAVARO, LAURA I. L.; BALCAO, VICTOR M.; ROCHA, LILIAM K. H.; SILVA, ERICA C.; OLIVEIRA JR, JOSE M.; VILA, MARTA M. D. C.; TUBINO, MATTHIEU. Physicochemical Characterization of a Crude Anthocyanin Extract from the Fruits of Jussara (Euterpe edulis Martius): Potential for Food and Pharmaceutical Applications. Journal of the Brazilian Chemical Society, v. 29, n. 10, p. 2072-2088, OCT 2018. Web of Science Citations: 6.
HARADA, LILIAM K.; PEREIRA, JORGE F. B.; CAMPOS, WELIDA F.; SILVA, ERICA C.; MOUTINHO, CARLA G.; VILA, MARTA M. D. C.; OLIVEIRA, JR., JOSE M.; TEIXEIRA, JOSE A.; BALCAO, VICTOR M.; TUBINO, MATTHIEU. Insights into Protein-Ionic Liquid Interactions Aiming at Macromolecule Delivery Systems. Journal of the Brazilian Chemical Society, v. 29, n. 10, p. 1983-1998, OCT 2018. Web of Science Citations: 4.
HARADA, LILIAM K.; SILVA, ERICA C.; CAMPOS, WELIDA F.; DEL FIOL, FERNANDO S.; VILA, MARTA; DABROWSKA, KRYSTYNA; KRYLOV, VICTOR N.; BALCAO, VICTOR M. Biotechnological applications of bacteriophages: State of the art. MICROBIOLOGICAL RESEARCH, v. 212, p. 38-58, 2018. Web of Science Citations: 13.
ROCHA, LILIAM K. H.; FAVARO, LAURA I. L.; RIOS, ALESSANDRA C.; SILVA, ERICA C.; SILVA, WELIDA F.; STIGLIANI, TATIANE P.; GUNGER, MARIANA; LIMA, RENATA; OLIVEIRA, JR., JOSE M.; ARANHA, NORBERTO; TUBINO, MATTHIEU; VILA, MARTA M. D. C.; BALCAO, VICTOR M. Sericin from Bombyx mori cocoons. Part I: Extraction and physicochemical-biological characterization for biopharmaceutical applications. Process Biochemistry, v. 61, p. 163-177, OCT 2017. Web of Science Citations: 8.

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