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Role of orexin neurons as mediators of the central effects induced by growth hormone

Grant number: 16/20897-3
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2017
Effective date (End): December 31, 2019
Field of knowledge:Biological Sciences - Physiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Jose Donato Junior
Grantee:Frederick Wasinski
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Orexins, also known as hypocretin, neuropeptides are responsible for regulating arousal, wakefulness, appetite, blood sugar control, and other physiological processes. They are found in neurons of the lateral hypothalamus and have neuroexcitatory effects. The lack of orexin in the brain is associated with narcolepsy in humans and animals, thus indicating its importance in the sleep-wake control. Interestingly, the growth hormone (GH) also modulates various biological functions controlled by orexins neurons. For example, GH-deficient individuals have severe metabolic disorders, such as sleep disturbance, hyperphagia, changes in glycemic control, obesity, among others. They feature changes in attention, wakefulness and sleep. However, a possible interaction between GH and orexin has never been directly studied. Thus, in this project, we formulated a hypothesis that GH may act directly on the orexin neurons, supporting neuroanatomical for many of the dysfunctions presented in GH deficiency, mediating the change in the functioning of orexin neurons. To investigate a possible interrelationship between GH and orexin neurons, we use mice that have GH receptor deficiency only in orexin neurons (GHR flox / flox orexin Cre). In this way, we can study the effects of GH exclusively on these neurons without affecting other cell populations with the GH receptor. The evolution of the weight of these animals will be monitored until adulthood, when they will be housed in individual boxes and tested for metabolic assessment, glucose and food intake. Subgroups will be subjected to food restriction and exercise along with its controls to study physiological situations in which GH secretion is high. From these experiments, we hope to better understand the mechanics involved in the communication between growth hormone and orexins neurons. Our data has the potential to identify the specific neuronal circuit involved in many of the effects of GH. (AU)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
QUARESMA, PAULA G. F.; DOS SANTOS, WILLIAN O.; WASINSKI, FREDERICK; METZGER, MARTIN; DONATO JR, JOSE. Neurochemical phenotype of growth hormone-responsive cells in the mouse paraventricular nucleus of the hypothalamus. JOURNAL OF COMPARATIVE NEUROLOGY, SEP 2020. Web of Science Citations: 0.
CAMPOS, ANA M. P.; TEIXEIRA, PRYSCILA D. S.; WASINSKI, FREDERICK; KLEIN, MARIANNE O.; BITTENCOURT, JACKSON C.; METZGER, MARTIN; DONATO, JR., JOSE. Differences between rats and mice in the leptin action on the paraventricular nucleus of the hypothalamus: Implications for the regulation of the hypothalamic-pituitary-thyroid axis. Journal of Neuroendocrinology, AUG 2020. Web of Science Citations: 1.
WASINSKI, FREDERICK; PEDROSO, JOAO A. B.; DOS SANTOS, WILLIAN O.; FURIGO, ISADORA C.; GARCIA-GALIANO, DAVID; ELIAS, CAROL F.; LIST, EDWARD O.; KOPCHICK, JOHN J.; SZAWKA, RAPHAEL E.; DONATO JR, JOSE. Tyrosine Hydroxylase Neurons Regulate Growth Hormone Secretion via Short-Loop Negative Feedback. JOURNAL OF NEUROSCIENCE, v. 40, n. 22, p. 4309-4322, MAY 27 2020. Web of Science Citations: 0.
WASINSKI, FREDERICK; FURIGO, ISADORA C.; TEIXEIRA, PRYSCILA D. S.; RAMOS-LOBO, ANGELA M.; PERONI, CIBELE N.; BARTOLINI, PAOLO; LIST, EDWARD O.; KOPCHICK, JOHN J.; DONATO JR, JOSE. Growth Hormone Receptor Deletion Reduces the Density of Axonal Projections from Hypothalamic Arcuate Nucleus Neurons. Neuroscience, v. 434, p. 136-147, MAY 10 2020. Web of Science Citations: 0.
QUARESMA, PAULA G. F.; TEIXEIRA, PRYSCILA D. S.; FURIGO, ISADORA C.; WASINSKI, FREDERICK; COUTO, GISELE C.; FRAZAO, RENATA; LIST, EDWARD O.; KOPCHICK, JOHN J.; DONATO JR, JOSE. Growth hormone/STAT5 signaling in proopiomelanocortin neurons regulates glucoprivic hyperphagia. Molecular and Cellular Endocrinology, v. 498, DEC 1 2019. Web of Science Citations: 2.
TEIXEIRA, PRYSCILA D. S.; WASINSKI, FREDERICK; LIMA, LEANDRO B.; FRAZAO, RENATA; BITTENCOURT, JACKSON C.; DONATO JR, JOSE. Regulation and neurochemical identity of melanin-concentrating hormone neurones in the preoptic area of lactating mice. Journal of Neuroendocrinology, v. 32, n. 2 DEC 2019. Web of Science Citations: 0.
FREDERICK WASINSKI; RENATA FRAZÃO; JOSE DONATO JR. Effects of growth hormone in the central nervous system. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 63, n. 6, p. 549-556, Nov. 2019. Web of Science Citations: 1.
FURIGO, ISADORA C.; MELO, HELEN M.; LYRA E SILVA, NATALIA M.; RAMOS-LOBO, ANGELA M.; TEIXEIRA, PRYSCILA D. S.; BUONFIGLIO, DANIELLA C.; WASINSKI, FREDERICK; LIMA, ELIANA R.; HIGUTI, ELIZA; PERONI, CIBELE N.; BARTOLINI, PAOLO; SOARES, CARLOS R. J.; METZGER, MARTIN; DE FELICE, FERNANDA G.; DONATO, JR., JOSE. Brain STAT5 signaling modulates learning and memory formation. Brain Structure & Function, v. 223, n. 5, p. 2229-2241, JUN 2018. Web of Science Citations: 9.
RAMOS-LOBO, ANGELA M.; FURIGO, ISADORA C.; TEIXEIRA, PRYSCILA D. S.; ZAMPIERI, THAIS T.; WASINSKI, FREDERICK; BUONFIGLIO, DANIELLA C.; DONATO, JR., JOSE. Maternal metabolic adaptations are necessary for normal offspring growth and brain development. PHYSIOLOGICAL REPORTS, v. 6, n. 5 MAR 2018. Web of Science Citations: 4.

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