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Cynara scolymus extract (artichoke): antimicrobial action on anaerobic bacteria of dentistry importance

Grant number: 16/10204-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2016
Effective date (End): November 30, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Luciane Dias de Oliveira
Grantee:Marcelle Simões Coelho
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The use of plant extracts with antimicrobial action can bring numerous benefits to dental therapy, however, there are few studies evaluating this action on anaerobic microorganisms involved in oral infections. The aim of this study is to evaluate in vitro the antimicrobial action of glycolic artichoke extract (Cynara scolymus) on the anaerobic bacteria Porphyromonas gingivalis, Porphyromonas endodontalis, Parvimonas micra, Prevotella intermedia and Fusobacterium nucleatum. For the planktonic form, the microdilution broth method is used, according to the Clinical and Laboratory Standards Institute (CLSI) to determine the minimum inhibitory concentrations (MIC) and minimal microbicidal (CMM). For biofilms standardized suspensions (107 cells/mL) are added to microplate wells and after 48 h at 37°C (anaerobic chamber) under agitation will be treated with the extract for 5 min and 24 hs. Will include a positive control group (physiological solution, NaCl) and negative control group (chlorhexidine 0,12%), totaling six groups (n=10) analyzed for each bacteria. Then, biofilms must be broken with ultrasonic homogenizer, the suspensions are diluted and plated on Reinforced Clostridial Medium (RCM). After 48 or 72 h (37 ° C in an anaerobic chamber), the colony forming units are counted per milliliter (CFU/mL) and the values will be converted to log10. The data will be statistically analyzed by ANOVA and Tukey Test (p d 0.05). (AU)