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Microscopic analysis of different Phakopsora species on Arabidopsis thaliana: a model to investigate fungal colonization

Grant number: 16/25111-8
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 20, 2017
Effective date (End): August 30, 2017
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Lilian Amorim
Grantee:Isabela Vescove Primiano
Supervisor abroad: Ulrich Schaffrath
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Local de pesquisa : RWTH Aachen University, Germany  
Associated to the scholarship:15/26108-8 - Aggressiveness of Phakopsora: a comparative analysis of grapevine and soybean rusts with emphasis to histopathology and epidemiology, BP.DR

Abstract

Asian grapevine leaf rust (Phakopsora euvitis) and Asian soybean rust (Phakopsora pachyrhizi), both of the genus Phakopsora, are highly agressive on their host plant. Although Asian grapevine leaf rust presents symptoms similar to Asian soybean rust it seems to behave different than other rusts, i.e. showing high frequence of pustules on leaves occurring concomitantly with host tissue necrosis and leading to premature defoliation. This reduction in photosynthesis contributes to yield losses. Comparative studies of infection, colonization and symptoms development are important to understand the host-pathogen interactions and its relation with agressiveness. Thus, epidemiological studies are being developed (FAPESP 2015/26108-8) to compare and quantify rusts of the genus Phakopsora (P. euvitis e P. pachyrhizi) with bean rust (Uromyces appendiculatus), the latter being considered as a control treatment for comparison. However, only few investigations about characterization and quantification of host tissue colonized by P. euvits have been done using microscopic techiniques. With the purpose of better understanding the mechanisms involved in infection and colonization processes of plant - P. euvits interaction, microscopic analyses will be conducted to investigate and to quantify fungal colonization with different staining techiniques, including fluorescence and confocal microscopy, in mutants of Arabidopsis. Protocols will be adapted to host plants of each causal agent. These analysis will be supervised by professor Dr. Ulrich Schaffrath, at the Department of Plant Physiology of RWTH Aachen University, Germany. This research group has experience in the area of microscopy of Phakopsora pachyrhizi interacting in host (soybeans and Arabidopsis mutants) and nonhost (wild-type Arabidopsis) situations.