Cutaneous lupus erythematosus: histological, immunohistochemical and direct immunofluorescence comparative analysis between patients with and without systemic lupus erythematosus, according to two classifications

• Grant number: 16/15336-2
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2017
• Effective date (End): January 31, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal researcher: Maria Letícia Cintra
• Grantee: Larissa Juliana Batista da Silva
• Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Lupus erythematosus (LE) can develop purely cutaneous lesions to potentially fatal systemic disease. Cutaneous forms are classified as acute, subacute, chronic and tumid cutaneous LE (CLE), according to clinical, histological and laboratory criteria. The analysis of the influence of systemic factors, at the skin site, on the phenotypic expression of inflammatory and anti-inflammatory markers, can add information to the knowledge of the pathogenesis of the disease.The objective of this project is to correlate the results of the analysis of the direct immunofluorescence test and immunohistochemical expression of inflammatory and anti-inflammatory markers, in biopsy specimens of patients with LEC, with the presence or absence of systemic disease, according to the two following classification systems: the American College of Rheumatology (1982) and the International Collaborating Clinics 2012. Using these two methods criteria, the same patient may be classified either as having systemic disease and only CLE.59 skin biopsy samples with CLE, which were previously analyzed by histological and immunohistochemical techniques to assess the inflammatory, anti-inflammatory and cytotoxic T markers expression. The results have previously been tabulated and will be used for this project. For this project, we will collect the clinical data from these patients charts and record them in a protocolar way. After, we will fill up these results in a excel chart in order to correlate them with the immunophenotypic and direct immunofluorescence findings so far collected and tabulated. Each of the histologic, immunophenotypic and direct immunofluorescence findings will be compared with the clinical criteria and classification used for systemic lupus. (AU)