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Chemical and biotechnological study of Sinningia (Gesneriaceae) and evaluation of photoinduzed biological effect for PDT

Grant number: 16/06407-3
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): March 01, 2017
Effective date (End): February 29, 2020
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Marcos José Salvador
Grantee:Alessandra Freitas Serain
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):17/19523-4 - Mass spectrometry imaging technique to study drug distribution in different tumor tissue, BE.EP.DD

Abstract

The use of natural products dates back to ancient times and many are known to treat various diseases and is an inspiration for medicinal chemistry and a valuable source for the production of drugs due to the enormous diversity of chemical substance classes and distinct functions. Worldwide, and particularly in Brazil, holder of a fantastic biodiversity, only a small fraction of these organisms has been investigated and is harnessed technologically. With the improvement of analytical techniques such as Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS) and metabolomics approaches and dereplication, for distinguishing novel molecules of already described, studied the number of agencies has increased, especially for plants. This project aims to quiímico and biotechnological study Sinningia (Gesneriaceae) and the evaluation of the biological effect photoinduced for use in PDT. To do so will seek to study the metabolomic profile of plant species of Gesneriaceae family, which features plants with importance in terms of biological and chemical diversity activity, Sinningia gender (Sinningia schiffneri Fritsch, Sinningia mauroana Chautems and Sinningia magnificent (Otto & A . Dietr.) Wiehler) plants in nature, employing procedures MS, NMR or chromatography dereplication approaches for isolating bioactive compounds from the extracts and carry evaluation of the photoinduced biological effect in cell lines for use in Photodynamic Therapy (PDT). In the first stage of the study, chemical profiles will be obtained in order to identify some components for dereplication directly in crude extracts and in the second step, the isolation and chromatographic procedures employing structural identification using spectroscopic analysis (UV, IR, NMR (1D and 2D) and MS) in phytochemical study guided by the absorption of 400 to 800 nm region, the photochemical efficiency and the bioassays to evaluate the photoinduced biological effect in cell lines for use in Photodynamic Therapy (PDT). For fractionation the phytochemical study, from dereplication study, evaluating the absorption in the UV-visible, the evaluation of photochemical efficiency and bioactivity will be chosen two species and the most promising extracts these parameters and amount of mass to continue the study to the isolation of substances capable of triggering photoinduced biological effect. In biotechnology study will proceed to the development of cell culture and/or plantlets in vitro of most promising plant species in the bioassay, in order, beyond the phytochemical study, the handling of physical and chemical factors for the production of secondary metabolites, focusing on quinone derivatives documented in literature for Sinningia species and give examples of substances such as hypericin with application in PDT, and proceeding up comparative study of bioactive metabolites in cell cultures and those obtained from the fresh plant parts. This is an issue not much explored in the national scene and, although few published data on photoactive molecules for use in PDT obtained from Brazilian species, these molecules and phyto-derived can be valuable because of the potential applications of PDT as a therapeutic resource. (AU)