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Search for the gene coding for the amidotransferase connecting subunit in humans and the study of the function of MSC6 in yeast

Grant number: 16/25907-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2017
Effective date (End): February 28, 2018
Field of knowledge:Biological Sciences - Genetics
Principal Investigator:Mario Henrique de Barros
Grantee:Maria Antônia Kfouri Martins Soares
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID

Abstract

mRNA translation requires the correct association of the tRNA with the cognate aminoacid. This process is carried by a specific aminoacyl-tRNA synthase. Some bacteria and organelles do not have the complete of aminoacyl tRNA synthases and extra transamidation reactions are therefore required for the aminoacid inespecifically associated to the tRNA. For instance this process occurs in the maturation of Glu-tRNAQ into Gln-tRNAQ.Bacterial GatCAB amidotransferases are responsible for the transamidation of mischarged glutamyl-tRNAGln into glutaminyl-tRNAGln. Mitochondria also have a multienzymatic complex necessary for the transamidation of glutamyl-tRNAGln. Gtf1p, Qrs1p and Pet112p are considered constituents of mitochondrial GatFAB amidotransferase. Our aim here is to identify the human gene ortologus to yeast GTF1 through functional complementation assays using human cDNA libraries . We also intend to proceed in the caracterization of MSC6 , a qrs1 multicopy supressor, studying its involvment in mitochondrial translation. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIBEIRO FRANCO, LETICIA VELOSO; MODA, BRUNO S.; SOARES, MARIA A. K. M.; BARROS, MARIO H. Msc6p is required for mitochondrial translation initiation in the absence of formylated Met-tRNA(fMet). FEBS Journal, v. 286, n. 7, p. 1407-1419, APR 2019. Web of Science Citations: 0.

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