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Electrospun PVA-GSNO fibers: preparation and biological activity

Grant number: 17/04622-7
Support type:Scholarships abroad - Research Internship - Scientific Initiation
Effective date (Start): August 07, 2017
Effective date (End): December 06, 2017
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal researcher:Marcelo Ganzarolli de Oliveira
Grantee:Leonardo Pinheiro Giglio
Supervisor abroad: Alexander N. Zelikin
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Research place: Aarhus University, Denmark  
Associated to the scholarship:16/07741-4 - Polyethylene terephthalate mesh impregnated with polynitrosated polyester for topical nitric oxide release, BP.IC


Polymers capable of releasing nitric oxide (NO) in a controlled manner are currently an important class of materials for biomedical applications. Among the hydrophilic polymers used in the biomedical field, polyvinyl alcohol ([CH2CHOH]n, PVA) offers several advantages including easy processability and excellent biocompatibility (FDA approved for clinical use in humans). Preparation of PVA fibbers trough electrospinning can lead to an enormous increase in surface area, in addition to providing a highly porous material that may allows cell growth, enhancing its potential application for site-localized generation of NO. The group of Prof. Alexander N. Zelikin at the University of Aarhus, Denmark, demonstrated that PVA can be synthesized via reversible addition fragmentation chain transfer (RAFT), which allows the modulation and control of chain size and polymer structure, such as the inclusion of other functional groups at the ends of the polymer chains. In addition, functionalization of PVA with amino group (-NH2), allows the attachment of other specific groups to PVA via carbodiimide reaction. In this project, we will use RAFT polymerization to synthesize PVA with end-caped NH2 groups. PVA-NH2 will be subsequently reacted with glutathione (a sulphydryl-containing tripeptide), generating PVA-GSH. The PVA-GSH solution will be processed into fibers by electrospinning. The electrospun PVA-GSH fibbers will be further S-nitrosated, to generate PVA-GSNO. Free GSNO is a well-known endogenous NO donor molecule, therefore, PVA-GSNO is expected to exert the same biological activities of GSNO. The biological activity of this new biomaterial will be tested against HeLa cells. The dose-response results of cell viability for PVA-GSNO fibbers with different SNO charges will be correlated with their NO release profiles measured by chemiluminescence.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PICHETH, GUILHERME F.; DA SILVA, LAURA C. E.; GIGLIO, LEONARDO P.; PLIVELIC, TOMAS S.; DE OLIVEIRA, MARCELO G.. S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release. Journal of Colloid and Interface Science, v. 576, p. 457-467, . (18/14142-5, 17/04622-7, 16/02414-5, 17/19253-7)
GIGLIO, LEONARDO P.; PICHETH, GUILHERME F.; LOVSCHALL, KAJA BORUP; ZELIKIN, ALEXANDER N.; DE OLIVEIRA, MARCELO G.. S-nitrosothiol-terminated poly(vinyl alcohol): Nitric oxide release and skin blood flow response. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 98, p. 41-49, . (17/04622-7, 16/02414-5, 17/19253-7)

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