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Biodegradable and pH-responsive polymersomes for gene and protein delivery

Grant number: 16/23844-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2017
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Fernando Carlos Giacomelli
Grantee:Lindomar Jose Calumby Albuquerque
Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
Associated scholarship(s):17/11261-0 - Synthesis of PHPMA-b-PDPA, PEO-b-PDPA and RGD-PHPMA-b-PDPA conjugates with further engineering of pH-responsive polymersomes towards the encapsulation and delivery of hydrophilic biomacromolecules, BE.EP.DR


The project focuses on the manufacturing of polymeric vesicles (polymersomes) for gene and protein encapsulation and delivery. The first step aims the production and structural characterization of assemblies produced from the block copolymers PEO45-b-PCL118, PEO45-b-PLA174, PHPMAm-b-PDPAn and PEOm-b-PDPAn. The synthesis of the PDPA-based block copolymers is intended to be performed at the Centre of BioMedical Polymers (Czech Republic) during a BEPE internship. The structural characterization will be performed essentially via scattering techniques (DLS, SLS, ELS, SAXS) and imaging (TEM). Subsequently, the encapsulation and release of biomacromolecules (proteins and nucleic acid) will be investigated and further, interaction of the polymeric vesicles with biological system (cells) will be evaluated via MTT cytotoxicity, cellular uptake and intracellular release experiments. Similar investigations will be performed by using the block copolymer functionalized with the peptide sequence RGD (RGD-PHPMAm-b-PDPAn). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALBUQUERQUE, LINDOMAR J. C.; ALAVARSE, ALEX C.; CARLAN DA SILVA, MARIA C.; ZILSE, MORGANA S.; BARTH, MAITE T.; BELLETTINI, ISMAEL C.; GIACOMELLI, FERNANDO C. Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression. Macromolecular Bioscience, v. 18, n. 2 FEB 2018. Web of Science Citations: 3.
BATISTA, CARIN C. S.; ALBUQUERQUE, LINDOMAR J. C.; DE ARAUJO, IRIS; ALBUQUERQUE, BRUNNO L.; DA SILVA, FERNANDA D.; GIACOMELLI, FERNANDO C. Antimicrobial activity of nano-sized silver colloids stabilized by nitrogen-containing polymers: the key influence of the polymer capping. RSC ADVANCES, v. 8, n. 20, p. 10873-10882, 2018. Web of Science Citations: 8.

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