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The role of microRNAs and microvesicles in retinal degeneration animal model

Grant number: 17/07699-0
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2017
Effective date (End): April 30, 2019
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Carolina Beltrame Del Debbio
Grantee:Lorena Teixeira Frasson
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:15/24001-1 - MicroRNAs role in retinal stem cells regulation, AP.JP

Abstract

The increase in human life longevity favored the increase of the incidence of chronic-degenerative diseases, among them the visual deficiency. Since neuronal cells of the mammalian retina are not capable to spontaneous regeneration, pathologies leading to tissue degeneration and blindness are not clinically curable. For that, the hope for the recovery of visual acuity lies in the so-called Regenerative Cell Therapy, a research area that uses stem cells for tissue regeneration. Ciliary Epithelium, a tissue located near the retina, is a promising source of retinal stem cells. Although the studies are favorable to its clinical use, the non-permissive microenvironment for regeneration, the presence of inhibitory factors and the low efficiency in cellular reprogramming prevent the development of a therapy at this time.This project aims to evaluate the presence of microvesicles structures in cells from the Ciliary Epithelium (structures known to carry small RNAs involved in inhibiting the regenerative capacity of the retina of mammals), as well as their release, content analysis, cell incorporation, and its effect on regenerative capacity in the retina from normal animals and retinal degeneration animal model. Understanding these mechanisms may favor a possible retinal regenerative therapy or a complement to the currently available therapies. (AU)