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Monitoring of infectious contents, endotoxic and gene presence of resistance to antibiotics of symptomatic and asymptomatic primary infections during endodontic therapy

Grant number: 16/23950-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2017
Effective date (End): May 31, 2021
Field of knowledge:Health Sciences - Dentistry - Endodontics
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Brenda Paula Figueiredo de Almeida Gomes
Grantee:Augusto Rodrigues Lima
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated research grant:15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections, AP.TEM
Associated scholarship(s):18/09270-4 - Microbiological profile analysis using Nanopore technology and characterization of S. mutans isolated from primary endodontic infections, BE.EP.DR

Abstract

Teeth with pulp necrosis, painful symptomatology and periapical bone destruction tend to be colonized by anaerobic and predominantly Gram-negative microbiota. The main virulence factor of Gram-negative bacteria is represented by the release of endotoxins (LPS) present in the outer membrane of the bacterial cell wall. With the indiscriminate use of antimicrobial agents, these bacteria have developed resistance to them. Thus, the present study aims to: a) To verify the susceptibility of the microbiota of symptomatic and asymptomatic infected root canals to the chemical-mechanical preparation and to the use of intracanal medication for 30 days by counting the colony forming units (CFU). B) To monitor the microbiota composition of symptomatic and asymptomatic infected root canals, and of the periapical abscesses during endodontic therapy, using the checkerboard technique. C) To characterize the cultivable microbiota by Next Generation Sequencing (NGS) in cases of symptomatic and asymptomatic infections, and correlate them with the LPS levels. D) To analyze the effect of the chemical-mechanical preparation and the intracanal medication in the reduction of endotoxin levels of the root canal. E) To monitor the presence of 14 antibiotic resistance genes at different phases of endodontic treatment. F) To correlate these findings with the clinical signs and symptoms presented by the patients. Patients with a need for endodontic intervention due to the pulpal necrosis with the presence or absence of symptomatology will be selected. Monitoring of the stages of endodontic treatment will be done microbiologically through Checkerboard and CFUs counting; and trough the quantification of LPS levels and antibiotic resistance genes.The data will be tabulated and statistically analyzed. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, AUGUSTO R.; GANGULY, TRIDIB; WALKER, ALEJANDRO R.; ACOSTA, NATALIA; FRANCISCO, PRISCILA A.; PILEGGI, ROBERTA; LEMOS, JOSE A.; GOMES, BRENDA P. F. A.; ABRANCHES, JACQUELINE. Phenotypic and Genotypic Characterization of Streptococcus mutans Strains Isolated from Endodontic Infections. JOURNAL OF ENDODONTICS, v. 46, n. 12, p. 1876-1883, DEC 2020. Web of Science Citations: 0.
LIMA, AUGUSTO RODRIGUES; HERRERA, DANIEL RODRIGO; FRANCISCO, PRISCILA AMANDA; PEREIRA, ANDREA CARDOSO; LEMOS, JOSE; ABRANCHES, JACQUELINE; GOMES, BRENDA P. F. A. Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals. CLINICAL ORAL INVESTIGATIONS, NOV 2020. Web of Science Citations: 0.

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