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Design and construction of novel bioreporter strains to identify alternative DSF receptors in Xanthomonas citri subsp. citri

Grant number: 17/10517-1
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): October 01, 2017
Effective date (End): March 31, 2018
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Jesus Aparecido Ferro
Grantee:Juan Carlos Caicedo Cepeda
Supervisor abroad: Leo Eberl
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Local de pesquisa : University of Zurich (UZH), Switzerland  
Associated to the scholarship:15/22473-3 - Identification of alternative sensor(s) for DSF autoinducer, pathogenicity regulator in Xanthomonas citri subsp. citri, BP.PD

Abstract

Xanthomonas citri subsp. citri (Xcc) is the causal agent of citrus canker, a disease that affects almost all types of citrus crops. Production of particular pathogenicity factors in Xcc is coordinated by rpf gene cluster, which encodes elements of a cell-cell communication system called Quorum Sensing (QS). QS in Xcc is mediated by DSF (Diffusible Signal Factor), synthesis and perception of DSF are dependent on several Rpf proteins. Detailed analysis of the regulatory functions of the Rpf proteins have suggested the presence of several DSF sensor proteins other than RpfC. This proposal aims to design and to construct a DSF biosensor strains, to use it as molecular tools to identify alternative DSF receptors in Xcc, in order to understand mechanism of virulence regulation in better detail and thus find new targets for citrus canker treatment.