Resistance to insecticide in Euchistus heros (Hemiptera: Pentatomidae): genetic diversity and association mapping of resistance genes

• Grant number: 17/02393-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2017
• End date: July 10, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Celso Omoto
• Grantee: Erick Mauricio Goes Cordeiro
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 18/20668-0 - Development of molecular markers for early detection of insecticide resistance in Spodoptera frugiperda and Euschistus heros using transcriptome and whole genome sequencing analyses, BE.EP.PD

Abstract

The popularization of Bt soybeans strains- primarily used to control lepidopterans larvae -, the environmental changes caused by the expansion of cropping areas, and climate changes will change the status of E. heros and other pests from secondary pests to primary pest and eventually will become the primary focus of the pest management in soybean crops. For the efficient management of E. hero's population levels, chemical control using insecticides is widely used and possible complications derived from its usage must be addressed. Problems such as the rapid evolution of resistance to insecticide molecules, the range expansion of the pest to other areas, the dispersion of resistance gene might make E. heros a real concern to Brazilian agribusiness. Next Generation Sequencing (NGS) applied to population genomics is nowadays an important tool for the characterization of genetic diversity helping us to understand gene flow pattern in a landscape. The selection of environmental and climatic variables can shed light on the phenomenon of local adaptation. Popular approaches using quantitative tools such as association mapping can link phenotypes such as insecticide resistance to specific markers are now feasible and considerably simpler. Genotyping by Sequencing (GBS) has become very popular in recent years and has helped to elucidate important problems in many different research fields. The objectives of this project are to monitoring insecticide resistance in Brazilian populations, to characterize the genetic diversity in the main soybean producing regions in Brazil, to identify markers associated with different levels of resistance to insecticides, to identity of possible markers under selection, and to generate predictive models for the gene flow. The changes in allelic frequencies in soybean regions, the information regarding where are the major focuses of allelic diversity, the discovery of major gene flow routes, and identification of local adaptation will provide valuable information for decision-makers to define better tactics and management plans for E. heros.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, C. S.; CORDEIRO, E. M. G.; CORREA, A. S.. Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae). JOURNAL OF INSECT SCIENCE, v. 19, n. 5, OCT 31 2019. (17/02393-0)

ZUCCHI, I, MARIA; CORDEIRO, ERICK M. G.; WU, XING; LAMANA, LETICIA MARISE; BROWN, PATRICK J.; MANJUNATHA, SHILPA; GOMES VIANA, JOAO PAULO; OMOTO, CELSO; PINHEIRO, JOSE B.; CLOUGH, STEVEN J.. Population Genomics of the Neotropical Brown Stink Bug, Euschistus heros: The Most Important Emerging Insect Pest to Soybean in Brazil. FRONTIERS IN GENETICS, v. 10, OCT 31 2019. (17/02393-0)

FRANCISCHINI, FABRICIO J. B.; CORDEIRO, ERICK M. G.; DE CAMPOS, JAQUELINE B.; ALVES-PEREIRA, ALESSANDRO; GOMES VIANA, JOAO PAULO; WU, XING; WEI, WEI; BROWN, PATRICK; JOYCE, ANDREA; MURUA, GABRIELA; et al. Diatraea saccharalis history of colonization in the Americas. The case for human-mediated dispersal. PLoS One, v. 14, n. 7, JUL 24 2019. (17/02393-0)

SOARES, PATRICIA L.; CORDEIRO, ERICK M. G.; SANTOS, FREDERICO N. S.; OMOTO, CELSO; CORREA, ALBERTO S.. The reunion of two lineages of the Neotropical brown stink bug on soybean lands in the heart of Brazil. SCIENTIFIC REPORTS, v. 8, FEB 6 2018. (14/11495-3, 17/02393-0)

CORREA, ALBERTO S.; CORDEIRO, ERICK M. G.; OMOTO, CELSO. Agricultural insect hybridization and implications for pest management. Pest Management Science, v. 75, n. 11, p. 2857-2864, NOV 2019. (14/11495-3, 17/02393-0)

ZUCCHI, MARIA I.; CORDEIRO, ERICK M. G.; ALLEN, CLINT; NOVELLO, MARIANA; GOMES VIANA, JOAO PAULO; BROWN, PATRICK J.; MANJUNATHA, SHILPA; OMOTO, CELSO; PINHEIRO, JOSE BALDIN; CLOUGH, STEVEN J.. Patterns of Genome-Wide Variation, Population Differentiation and SNP Discovery of the Red Banded Stink Bug (Piezodorus guildinii). SCIENTIFIC REPORTS, v. 9, OCT 9 2019. (17/02393-0)

SILVA, CLEANE S.; CORDEIRO, ERICK M. G.; DE PAIVA, JULIA B.; DOURADO, PATRICK M.; CARVALHO, RENATO A.; HEAD, GRAHAM; MARTINELLI, SAMUEL; CORREA, ALBERTO S.. Population expansion and genomic adaptation to agricultural environments of the soybean looper, Chrysodeixis includens. EVOLUTIONARY APPLICATIONS, v. 13, n. 8, APR 2020. (17/02393-0)

GOES CORDEIRO, ERICK MAURICIO; SOARES, PATRICIA LIMA; ALVES, DENISE ARAUJO; CORREA, ALBERTO SOARES. Updating the saga of the small hive beetle (Aethina tumida): molecular inference of the origin of the South American invasion. Apidologie, v. 50, n. 3, p. 273-276, JUL 2019. (14/11495-3, 17/02393-0)

CORDEIRO, ERICK M. G.; PANTOJA-GOMEZ, LAURA M.; DE PAIVA, JULIA B.; NASCIMENTO, ANTONIO R. B.; OMOTO, CELSO; MICHEL, ANDREW P.; CORREA, ALBERTO S.. Hybridization and introgression between Helicoverpa armigera and H. zea: an adaptational bridge. BMC Evolutionary Biology, v. 20, n. 1, MAY 25 2020. (14/26212-7, 14/11495-3, 17/02393-0, 16/09159-0)