Modification and characterization of low-cost electrodes with material derived from 3-amino-4-hydroxybenzoic acid aiming the development of genosensor applied to the diagnosis of Zika virus

• Grant number: 17/07436-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2017
• End date: February 28, 2019
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Del Pilar Taboada Sotomayor
• Grantee: Rafael da Fonseca Alves
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 18/09596-7 - Molecularly Imprinted Biotin Polymer for Isolation and Detection of Zika-Specific Oligonucleotides Biotinylated Viruses with Lateral Flow and Electrochemical System, BE.EP.MS

Abstract

The Zika virus is a causative agent of infectious diseases that spreads in humans through the mosquitoes Aedes aegypti and Aedes albopictus, which are vectors of the virus. There are several transmission mechanisms, including mother to child transmission during pregnancy, which can lead to microcephaly in newborns and other birth defects. Efforts to monitor and control Zika virus infection globally are very limited and appear to be insufficient due to lack of accountability, lack of organization and unavailability of drugs, vaccines and diagnostic tools. In the context of the development of new diagnostic methodologies, the construction of biological sensors, also known as biosensors, has become a broad activity in the field of science, focusing mainly on issues of health care and problems with the environment. The development of these biosensors in the laboratory has been worked by several research groups, searching for specific devices, low cost, easy handling and preparation. The electrochemical techniques concentrate the greater number of works performed due to the ease in the process and the instrumentation, allowing to obtain fast and reliable results. In this sense, electrodes modified with conductive polymers are viable options, as they improve the analytical response and help in the immobilization of the biomolecules. Thus, this work aims at the study of the electrochemical and morphological properties of materials derived from the 3-amino-4-hydroxybenzoic acid monomer (AAHB) under the surface of graphite electrodes and graphite electrodes for the construction of a diagnostic genosensor Of the Zika virus. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, RAFAEL DA FONSECA; FRANCO, DIEGO LEONI; CORDEIRO, MARLI TENORIO; DE OLIVEIRA JUNIOR, ERNESTO MARQUES; FIREMAN DUTRA, ROSA AMALIA; TABOADA SOTOMAYOR, MARIA DEL PILAR. Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode. SENSORS AND ACTUATORS B-CHEMICAL, v. 296, OCT 1 2019. (17/07436-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Rafael da Fonseca. Modification and characterization of low cost electrodes with material derived of 3-amino-4-hydroxybenzoic acid aiming development the genosensor applied of the virus Zika diagnosis. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.