Comparative immunopathology of cetacean morbillivirus infection: histologic, immunohistochemical, and molecular studies

• Grant number: 17/02223-8
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): July 01, 2017
• Effective date (End): April 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Jose Luiz Catao Dias
• Grantee: Josué Díaz Delgado
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/01876-0 - Comparative immunopathology of cetacean morbillivirus infection: histologic, immunohistochemical, and molecular studies, BE.EP.PD

Abstract

Cetacean morbillivirus (CeMV; genus Morbillivirus, family Paramyxoviridae) has caused multipleoutbreaks of lethal disease in odontocetes and mysticetes worldwide. CeMV includes three wellcharacterized strains: porpoise morbillivirus, dolphin morbillivirus, and pilot whale morbillivirus(northern hemisphere), and three novel strains, one of them detected in Brazil and considered thefirst description in South America. Studies suggest there is different species-specific susceptibilityto CeMV infection, and bottlenose dolphins (Tursiops truncatus) and striped dolphins (Stenellacoeruleoalba) are among the most susceptible ones, with historical fatal epizootics. CeMV maycause severe respiratory, lymphoid, and neurologic disease in susceptible species, leading tostrandings and death. Four major presentations of CeMV-associated pathology (CeMV-AP) arecurrently recognized, which bear resemblance to Measles (MeV) and Distemper (CDV), the majormorbilliviral diseases in humans and dogs, respectively. Furthermore, studies have shown cytokineimbalance in TH1 and TH2 immune responses plays a major role in disease susceptibility andprogression in MeV- and CDV-infected individuals. No previous studies have evaluated the localimmunopathogenetic aspects in tissues other than peripheral blood in CeMV-AP. This study willemploy archival, formalin-fixed, paraffin-embedded and frozen tissues from organs with majorpathogenetic significance (brain, lung, and lymph nodes) of CeMV-positive bottlenose dolphins andstriped dolphins (central eastern Atlantic, Mediterranean Sea), and selected Brazilian species(southwestern Atlantic), as determined by RT-PCR analysis. A suit of lymphocytic, histiocytic andadditional immunohistochemical markers will be used to characterize the local immune responses.Additionally, the expression of TH1 and TH2 cytokines will be evaluated via RT-qPCR/PCR.Parallel histopathologic, immunohistochemical, and molecular analysis may provide insight intoimmunopathogenetic mechanisms of CeMV-AP, a disease with worldwide distribution and majorconservation significance.

Scientific publications

(34)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GROCH, KATIA R.; DIAZ-DELGADO, JOSUE; SANTOS-NETO, ELITIERI B.; IKEDA, JOANA M. P.; CARVALHO, RAFAEL R.; OLIVEIRA, RAISSA B.; GUARI, EMI B.; FLACH, LEONARDO; SIERRA, EVA; GODINHO, ANA I.; et al. The Pathology of Cetacean Morbillivirus Infection and Comorbidities in Guiana Dolphins During an Unusual Mortality Event (Brazil, 2017-2018). VETERINARY PATHOLOGY, v. 57, n. 6, p. 845-857, NOV 2020. (17/24335-2, 15/05043-5, 15/00735-6, 14/24932-2, 17/02223-8)

NAVAS-SUAREZ, P. E.; DIAZ-DELGADO, J.; FERNANDES-SANTOS, R. C.; TESTA-JOSE, C.; SILVA, R.; SANSONE, M.; MEDICI, E. P.; CATAO-DIAS, J. L.. Pathological Findings in Lowland Tapirs (Tapirus terrestris) Killed by Motor Vehicle Collision in the Brazilian Cerrado. Journal of Comparative Pathology, v. 170, p. 34-45, JUL 2019. (17/02223-8)

RODRIGUES, THAIS CARNEIRO S.; DIAZ-DELGADO, JOSUE; CATAO-DIAS, JOSE LUIZ; CARVALHO, JULIA DA LUZ; MARMONTEL, MIRIAM. Retrospective pathological survey of pulmonary disease in free-ranging Amazon river dolphin Inia geoffrensis and tucuxi Sotalia fluviatilis. DISEASES OF AQUATIC ORGANISMS, v. 131, n. 1, p. 1-11, OCT 16 2018. (17/02223-8)

DE AZEVEDO FERNANDES, NATALIA C. C.; GUERRA, JULIANA M.; DIAZ-DELGADO, JOSUE; CUNHA, MARIANA S.; SAAD, LEILA DEL C.; IGLEZIAS, SILVIA D.; RESSIO, RODRIGO A.; CIRQUEIRA, CINTHYA DOS SANTOS; KANAMURA, CRISTINA T.; JESUS, ISIS P.; et al. Differential Yellow Fever Susceptibility in New World Nonhuman Primates, Comparison with Humans, and Implications for Surveillance. Emerging Infectious Diseases, v. 27, n. 1, p. 47-56, JAN 2021. (17/02223-8)

ALVES-MOTTA, M. R.; LUZ-CARVALHO, V; NUNES-PINHEIRO, D. C. S.; GROCH, K. R.; GONCALVES-PEREIRA, L.; SANCHEZ-SARMIENTO, A. M.; SACRISTAN, C.; CATAO-DIAS, J. L.; DIAZ-DELGADO, J.. Facial Squamous Cell Carcinoma and Abdominal Peripheral Nerve Sheath Tumour with Rhabdomyoblastic Differentiation in a Rough-toothed Dolphin (Steno bredanensis). Journal of Comparative Pathology, v. 176, p. 122-127, APR 2020. (18/25069-7, 17/02223-8)

NAKAGUN, SHOTARO; DIAZ-DELGADO, JOSUE; GROCH, KATIA R.; KOBAYASHI, YOSHIYASU. Intrapancreatic accessory spleen in a harbor porpoise (Phocoena phocoena). JOURNAL OF VETERINARY MEDICAL SCIENCE, v. 80, n. 4, p. 701-705, APR 2018. (14/24932-2, 17/02223-8)

GROCH, KATIA R.; SANTOS-NETO, ELITIERI B.; DIAZ-DELGADO, JOSUE; IKEDA, JOANA M. P.; CARVALHO, RAFAEL R.; OLIVEIRA, RAISSA B.; GUARI, EMI B.; BISI, TATIANA L.; AZEVEDO, ALEXANDRE F.; LAILSON-BRITO, JOSE; et al. Guiana Dolphin Unusual Mortality Event and Link to Cetacean Morbillivirus, Brazil. Emerging Infectious Diseases, v. 24, n. 7, p. 1349-1354, JUL 2018. (14/24932-2, 15/00735-6, 17/02223-8)

DIAZ-DELGADO, J.; GOMES-BORGES, J. C.; MONTEIRO SILVEIRA, A.; EINHARDT-VERGARA, J.; GROCH, K. R.; CIRQUEIRA, C. S.; SANSONE, M.; GATTAMORTA, M. A.; MATUSHIMA, E. R.; CATAO-DIAS, J. L.. Primary Multicentric Pulmonary Low-grade Fibromyxoid Sarcoma and Chelonid Alphaherpesvirus 5 Detection in a Leatherback Sea Turtle (Dermochelys coriacea). Journal of Comparative Pathology, v. 168, p. 1-7, APR 2019. (14/24932-2, 17/02223-8, 15/00735-6)

NAVAS-SUAREZ, PEDRO ENRIQUE; DIAZ-DELGADO, JOSUE; MATUSHIMA, ELIANA REIKO; FAVERO, CINTIA MARIA; SANCHEZ SARMIENTO, ANGELICA MARIA; SACRISTAN, CARLOS; EWBANK, ANA CAROLINA; JOPPERT, ADRIANA MARQUES; BARBANTI DUARTE, JOSE MAURICIO; DOS SANTOS-CIRQUEIRA, CINTHYA; et al. A retrospective pathology study of two Neotropical deer species (1995-2015), Brazil: Marsh deer (Blastocerus dichotomus) and brown brocket deer (Mamma gouazoubira). PLoS One, v. 13, n. 6, JUN 7 2018. (17/02223-8, 15/04231-2)

CARVALHO, V. L.; GROCH, K. R.; CATAO-DIAS, J. L.; MEIRELLES, A. C. O.; SILVA, C. P. N.; MONTEIRO, A. N. B.; DIAZ-DELGADO, J.. Cerebral and cardiac congenital malformations in neonatal West Indian manatees (Trichechus manatus). Journal of Comparative Pathology, v. 166, p. 29-34, JAN 2019. (14/24932-2, 17/02223-8, 15/00735-6)

DIAZ-DELGADO, JOSUE; GROCH, KATIA R.; SIERRA, EVA; SACCHINI, SIMONA; ZUCCA, DANIELE; QUESADA-CANALES, OSCAR; ARBELO, MANUEL; FERNANDEZ, ANTONIO; SANTOS, ELITIERI; IKEDA, JOANA; et al. Comparative histopathologic and viral immunohistochemical studies on CeMV infection among Western Mediterranean, Northeast-Central, and Southwestern Atlantic cetaceans. PLoS One, v. 14, n. 3, MAR 20 2019. (15/00735-6, 15/05043-5, 17/02223-8, 17/24335-2, 14/24932-2)

GROCH, KATIA R.; BLAZQUEZ, DIANA N. H.; MARCONDES, MILTON C. C.; SANTOS, JOANA; COLOSIO, ADRIANA; DELGADO, JOSUE DIAZ; CATAO-DIAS, JOSE L.. Cetacean morbillivirus in Humpback whales' exhaled breath. TRANSBOUNDARY AND EMERGING DISEASES, OCT 2020. (19/11053-4, 15/00735-6, 17/02223-8, 15/05043-5)

SAMIRA COSTA-SILVA; CARLOS SACRISTÁN; OMAR GONZALES-VIERA; JOSUÉ DÍAZ-DELGADO; ANGÉLICA MARÍA SÁNCHEZ-SARMIENTO; JULIANA MARIGO; KÁTIA REGINA GROCH; VITOR LUZ CARVALHO; ANA CAROLINA EWBANK; ADRIANA CASTALDO COLOSIO; et al. Toxoplasma gondii em cetáceos do Brasil: estudo histopatológico e imuno-histoquímico. Revista Brasileira de Parasitologia Veterinária, v. 28, n. 3, p. 395-402, 2019-08-12. (17/02223-8)

MARIANI, DANIELA BUENO; GUIMARAES, JULIANA PLACIDO; BATISTA, RENATA GUEDES; BRUM, ANDREI; GROCH, KATIA REGINA; DIAZ-DELGADO, JOSUE; VERGARA PARENTE, JOCIERY EINHARDT. Asfixia por deslocamento laríngeo associado a consumo de presa de grande tamanho em boto-cinza (Sotalia guianensis), Brasil. Ciência Rural, v. 50, n. 2, 2020. (14/24932-2, 17/02223-8)

DE CARVALHO, M. P. N.; CUNHA, V, M. P.; KNOBL, T.; CIRQUEIRA, C. S.; DIAS-NETO, R. N.; SERAFINI, P. P.; CATAO-DIAS, J. L.; DIAZ-DELGADO, J.. Cardiac disease in the Spix Macaw (Cyanopsitta spixii): two cases. AUSTRALIAN VETERINARY JOURNAL, JUN 2021. (17/02223-8)

BIANCHI, MATHEUS V.; EHLERS, LUIZA P.; VARGAS, THAINA P.; LOPES, BRUNA C.; TAUNDE, PAULA A.; DE CECCO, BIANCA S.; HENKER, LUAN C.; VIELMO, ANDREIA; LORENZETT, MARINA P.; RIBOLDI, CAMILA I.; et al. Omphalitis, urachocystitis and septicemia by Streptococcus dysgalactiae in a southern right whale calf Eubalaena australis, Brazil. DISEASES OF AQUATIC ORGANISMS, v. 131, n. 3, p. 227-232, NOV 20 2018. (17/02223-8)

GROCH, KATIA R.; BLAZQUEZ, DIANA N. H.; MARCONDES, MILTON C. C.; SANTOS, JOANA; COLOSIO, ADRIANA; DELGADO, JOSUE DIAZ; CATAO-DIAS, JOSE L.. Cetacean morbillivirus in Humpback whales' exhaled breath. TRANSBOUNDARY AND EMERGING DISEASES, v. 68, n. 4, p. 1736-1743, JUL 2021. (15/00735-6, 19/11053-4, 17/02223-8, 15/05043-5)

NAVAS-SUAREZ, PEDRO ENRIQUE; SACRISTAN, CARLOS; KLUYBER, DANILO; YOGUI, DEBORA REGINA; ALVES, AMANDA CAROLINA; DALAZEN, GISLAINE TAIMARA; DIAZ-DELGADO, JOSUE; GUERRA, JULIANA MARIOTTI; DE AZEVEDO FERNANDES, NATALIA COELHO COUTO; RESSIO, RODRIGO ALBERGARIA; et al. Novel gammaherpesvirus associated with primary gastric T-cell lymphoma in a free-ranging giant armadillo in Brazil. TRANSBOUNDARY AND EMERGING DISEASES, JUN 2021. (18/25069-7, 17/02223-8)

GROCH, KATIA R.; CATAO-DIAS, JOSE L.; GROCH, KARINA R.; KOLESNIKOVAS, CRISTIANE K. M.; DE CASTILHO, V, PEDRO; MOREIRA, LUCIANA M. P.; BARROS, CECIL R. M. B.; MORAIS DE MEDEIROS, CAMILA R.; RENAULT-BRAGA, EDUARDO P.; SANSONE, MARCELO; et al. Pathologic findings and causes of death in southern right whales Eubalaena australis, Brazil. DISEASES OF AQUATIC ORGANISMS, v. 137, n. 1, p. 23-31, NOV 28 2019. (14/24932-2, 17/02223-8, 15/00735-6)

DUARTE-BENVENUTO, ARICIA; DOS SANTOS COSTA, PRISCILLA CARLA; JERDY, HASSAN; QUEIROZ DE CARVALHO, EULOGIO CARLOS; CATAO-DIAS, JOSE LUIZ; DIAZ-DELGADO, JOSUE. Spontaneous Primary Right Tibiotarsal Osteosarcoma with Pulmonary Metastasis in a Free-ranging Cory's Shearwater (Calonectris diomedea). JOURNAL OF WILDLIFE DISEASES, v. 56, n. 1, p. 243-246, JAN 2020. (17/02223-8)

ARREGUI, MARINA; BERNALDO DE QUIROS, YARA; SAAVEDRA, PEDRO; SIERRA, EVA; SUAREZ-SANTANA, CRISTIAN M.; ARBELO, MANUEL; DIAZ-DELGADO, JOSUE; PUIG-LOZANO, RAQUEL; ANDRADA, MARISA; FERNANDEZ, ANTONIO. Fat Embolism and Sperm Whale Ship Strikes. FRONTIERS IN MARINE SCIENCE, v. 6, JUL 3 2019. (17/02223-8)

DIAZ-DELGADO, JOSUE; GROCH, KATIA R.; RAMOS, HERNANI G. C.; COLOSIO, ADRIANA CASTALDO; ALVES, BRUNA FARIAS; PENA, HILDA F. J.; CATAO-DIAS, JOSE L.. Fatal Systemic Toxoplasmosis by a Novel Non-archetypal Toxoplasma gondii in a Bryde's Whale (Balaenoptera edeni). FRONTIERS IN MARINE SCIENCE, v. 7, JUN 3 2020. (14/24932-2, 17/02223-8)

DIAZ-DELGADO, J.; SANCHES, T. C.; CIRQUEIRA, C. S.; COIMBRA, A. A. C.; GUERRA, J. M.; OLIVARES, V.; DI LORETTO, C.; RESSIO, R. A.; IGLEZIAS, S.; FERNANDES, N. C. C. A.; et al. Multicentric cutaneous keratoacanthomas in a free-living marmoset (Callithrix sp.). JOURNAL OF MEDICAL PRIMATOLOGY, v. 47, n. 3, p. 205-208, JUN 2018. (17/02223-8)

DIAZ-DELGADO, JOSUE; GROCH, KATIE R.; RESSIO, RODRIGO; RISKALLAH, ISIS P. J.; SIERRA, EVA; SACCHINI, SIMONA; QUESADA-CANALES, OSCAR; ARBELO, MANUEL; FERNANDEZ, ANTONIO; SANTOS-NETO, ELITIERI; et al. Comparative Immunopathology of Cetacean morbillivirus Infection in Free-Ranging Dolphins From Western Mediterranean, Northeast-Central, and Southwestern Atlantic. FRONTIERS IN IMMUNOLOGY, v. 10, MAR 18 2019. (17/02223-8)

CARVALHO, M. P. N.; SANT'ANNA, S. S.; DIAZ-DELGADO, J.; GREGO, K. E.; GOGONE, I. C. V. P.; QUEIROZ-HAZARBASSANOV, N. G. T.; CATAO-DIAS, J. L.. Histopathological Findings in the Viscera of Malformed Neotropical Viperids. Journal of Comparative Pathology, v. 167, p. 73-81, FEB 2019. (17/02223-8)

DIAZ-DELGADO, J.; RESSIO, R.; GROCH, K. R.; CATAO-DIAS, J. L.. Immunohistochemical investigation of the cross-reactivity of selected cell markers in formalin-fixed, paraffin-embedded lymphoid tissues of Franciscana (Pontoporia blainvillei). VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, v. 200, p. 52-58, JUN 2018. (14/24932-2, 15/00735-6, 17/02223-8)

COUTO DE AZEVEDO FERNANDES, NATALIA COELHO; CUNHA, MARIANA SEQUETIN; GUERRA, JULIANA MARIOTTI; RESSIO, RODRIGO ALBERGARIA; CIRQUEIRA, CINTHYA DOS SANTOS; IGLEZIAS, SILVIA D'ANDRETTA; DE CARVALHO, JULIA; ARAUJO, EMERSON L. L.; CATAO-DIAS, JOSE LUIZ; DIAZ-DELGADO, JOSUE. Outbreak of Yellow Fever among Nonhuman Primates, Espirito Santo, Brazil, 2017. Emerging Infectious Diseases, v. 23, n. 12, p. 2038-2041, DEC 2017. (17/02223-8)

DIAZ-DELGADO, J.; GUERRA, J. M.; FERNANDES, N. C. C. A.; GONCALVES-SERRA, E.; MINOZZO, G. A.; DI LORETTO, C.; IGLEZIAS, S.; GROCH, K. R.; RESSIO, R.; KANAMURA, C.; et al. Spontaneous pulmonary adenosquamous carcinoma in a free-living black capuchin monkey (Sapajus nigritus). JOURNAL OF MEDICAL PRIMATOLOGY, v. 47, n. 2, p. 120-123, APR 2018. (17/02223-8)

DIAZ-DELGADO, J.; SANCHES, T. C.; DOS SANTOS-CIRQUEIRA, C.; COIMBRA, A. A. C.; GUERRA, J. M.; JOPPERT, A.; DI LORETTO, C.; RIZARDI, M. B.; SANSONE, M.; NAGAMORI, F. O.; et al. Hepatocellular carcinoma in a free-living marmoset (Callithrix sp.) with concomitant biliary trematodiasis. JOURNAL OF MEDICAL PRIMATOLOGY, v. 47, n. 2, p. 128-131, APR 2018. (17/02223-8)

GROCH, KATIA R.; DIAZ-DELGADO, JOSUE; SACRISTAN, CARLOS; OLIVEIRA, DENYIELIM E.; SOUZA, GABRIELA; SANCHEZ-SARMIENTO, ANGELICA M.; COSTA-SILVA, SAMIRA; MARIGO, JULIANA; CASTILHO, PEDRO V.; CREMER, MARTA J.; et al. Pulmonary and systemic fungal infections in an Atlantic spotted dolphin and a Bryde's whale, Brazil. DISEASES OF AQUATIC ORGANISMS, v. 128, n. 1, p. 73-79, MAR 22 2018. (14/24932-2, 15/00735-6, 17/02223-8)

GROCH, KATIA R.; GROCH, KARINA R.; KOLESNIKOVAS, CRISTIANE K. M.; DE CASTILHO, PEDRO V.; MOREIRA, LUCIANA M. P.; BARROS, CECIL R. M. B.; MORAIS, CAMILA R.; RENAULT-BRAGA, EDUARDO P.; SIERRA, EVA; FERNANDEZ, ANTONIO; et al. Cetacean morbillivirus in Southern Right Whales, Brazil. TRANSBOUNDARY AND EMERGING DISEASES, v. 66, n. 1, p. 606-610, JAN 2019. (17/02223-8, 15/00735-6, 14/24932-2, 15/05043-5)

GROCH, KATIA R.; JERDY, HASSAN; MARCONDES, MILTON C. C.; BARBOSA, LUPERCIO A.; RAMOS, HERNANI G. C.; PAVANELLI, LARISSA; FORNELLS, LUZ ALBA M. G.; SILVA, MARINA B.; SOUZA, GILIANE S.; KANASHIRO, MILTON M.; et al. Cetacean Morbillivirus Infection in a Killer Whale (Orcinus orca) from Brazil. Journal of Comparative Pathology, v. 181, p. 26-32, NOV 2020. (15/00735-6, 15/05043-5, 17/02223-8, 14/24932-2)

DE CARVALHO, M. P. N.; CUNHA, V, M. P.; KNOBL, T.; CIRQUEIRA, C. S.; DIAS-NETO, R. N.; SERAFINI, P. P.; CATAO-DIAS, J. L.; DIAZ-DELGADO, J.. Cardiac disease in the Spix Macaw (Cyanopsitta spixii): two cases. AUSTRALIAN VETERINARY JOURNAL, v. 99, n. 9, p. 402-407, SEP 2021. (17/02223-8)

NAVAS-SUAREZ, PEDRO ENRIQUE; SACRISTAN, CARLOS; DIAZ-DELGADO, JOSUE; YOGUI, DEBORA R.; ALVES, MARIO HENRIQUE; FUENTES-CASTILLO, DANNY; OSPINA-PINTO, CATALINA; ZAMANA, ROBERTA RAMBLAS; JEAN DESBIEZ, ARNAUD LEONARD; CATAO-DIAS, JOSE LUIZ. Pulmonary adiaspiromycosis in armadillos killed by motor vehicle collisions in Brazil. SCIENTIFIC REPORTS, v. 11, n. 1, JAN 11 2021. (18/25069-7, 17/02223-8)