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Cryopreservation: the crystals ice formation and their relation to the cell injuries

Grant number: 17/13245-2
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): July 31, 2017
Effective date (End): August 31, 2017
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal researcher:Elizabeth Romagosa
Grantee:Taís da Silva Lopes
Supervisor abroad: Michael A Menze
Home Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
Research place: University of Louisville (UofL), United States  
Associated to the scholarship:14/21215-8 - Cryopreservation of surubim-do-paraíba (Steindachneridion parahybae) oocites, BP.PD

Abstract

The benefits that cryopreservation of animal cells can provide are innumerable and undeniable. However, many difficulties still exist to establish efficient protocols, and in the case of fish oocytes, to guarantee their viability. In this way, studies are needed to deepen the knowledge about cryobiology of animal cells, the process of ice formation, and their impact on the injuries caused to cryopreserved tissues. To this end, the present Research Internship abroad is intended to provide contact with leading technologies used in human cryobiology studies, as well as to seek guidance from a reference team in the area of cryopreservation, to assist in the discussions of the articles being prepared about the Postdoctoral studies (in progress). These are the techniques developed in the PhD Michael A. Menze laboratory, suitable for application to fish oocytes: studies on mitochondrial bioenergy; isothermal calorimetry; cytotoxicity assays based on fluorescence or protein microscopy; and Raman confocal microspectroscopy (CRM). The results of the activities developed will be presented in the form of a report, and scientific articles will be completed and submitted for publication and dissemination of the results, with the help of the discussions elaborated during the internship.

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