Development of production process of baculovirus biopesticide by Sf9 insect cells in bioreactor

• Grant number: 17/06371-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2017
• End date: August 31, 2019
• Field of knowledge: Engineering - Chemical Engineering
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Aldo Tonso
• Grantee: Karina Klafke
• Host Institution: Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/08526-5 - Baculovirus production comparing STR and wave bioreactors, BE.EP.MS

Abstract

Several baculoviruses have been used as biological pesticides for the control of soybean caterpillar, an important pest of soybean plantations in Brazil, such as Anticarsia gemmatalis multiplenucleopolyhedrovirus (AgMNPV). Currently, AgMNPV is produced through caterpillar infection (in vivo) and the possibility of production by insect cell infection in culture (in vitro) is studied. To do so, it is necessary to determine the best cell culture conditions that will be employed in the production of the virus, in order to maximize the production of the viral polyhedra. In this direction, the present project has the objective of studying the process of baculovirus production in Sf-9 insect cells for the production of bioinsecticide. For this, important factors such as the growth of insect cells in bioreactors will be taken into account to obtain cultures with high cell density. The nutrient supply to the cells will be tested in different ways, through fed-batch cultures, in order to prolong the exponential growth phase, resulting in a higher cell concentration to be infected. The infection stage will also be studied, obtaining parameters to guarantee the virulence of the product (baculovirus polyhedra). Analyzes of viable cell growth, substrate consumption (glucose and glutamate) and lactate production will be performed to monitor cell development. Analyzing the experimental data, it is expected to better understand cell growth metabolism and thereby improve the infection process and cultures in bioreactors. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KLAFKE, KARINA; SANCHES, MARCIO MARTINELLO; SIHLER, WILLIAM; DE SOUZA, MARLINDA LOBO; TONSO, ALDO. Bioreactor Production Process of Spodoptera frugiperda multiple nucleopolyhedrovirus Biopesticide. PATHOGENS, v. 12, n. 8, p. 13-pg., 2023-08-01. (17/06371-1, 18/08526-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

KLAFKE, Karina. Development of the bioinsecticide production process in bioreactor for Spodoptera frugiperda MNPV in Sf-9 insect cells.. 2020. Master's Dissertation - Universidade de São Paulo (USP). Escola Politécnica (EP/BC) São Paulo.