Advanced search
Start date

Adjustments on males reproductive physiology of Astyanax altiparanae (Teleostei:Characidae) exposed to emerging compounds

Grant number: 17/07139-5
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2017
Effective date (End): July 31, 2019
Field of knowledge:Biological Sciences - Physiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Renata Guimarães Moreira Whitton
Grantee:Filipe Guilherme Andrade de Godoi
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach, AP.PFPMCG.TEM
Associated scholarship(s):18/10495-0 - Di-isononyl phthalate an emerging contaminant in freshwater - what are the risks for apoptotic and autophagyc processes in zebrafish gonads, BE.EP.MS


Environmental contamination via compounds like metals and pesticides has affected the biodiversity, mostly in the aquatic environments. These substances change the individuals' homeostasis and consequently the survival capacity of animals. A new group of substances called emerging compounds has been reported in water resources and some effects identified, including endocrine changes. Caffeine and diclofenac, mainly present, caused alterations in the vertebrates reproductive physiology as well as modifications in hepatic, kidney, gills morphology of teleosts. Based on the effects of emerging compounds in the physiology of aquatic organisms, and the knowledge of the importance of reproduction on the species safety, the main objective of the present study is to evaluate if diclofenac and caffeine, together or separated, can act as endocrine disrupters (ED) on the males reproductive physiology of Astyanax altiparanae. This biological model is broadly distributed in the brazilian aquatic environments and this species has been study due to its ecological importance in the ecosystems and in the socioeconomically development. Males are relevant bioindicators of ED presence in impaired environments, considering the mainly presence of estrogenic compounds, due to the presence of genes related to vilelogenin (Vtg) synthesis. In this context, the gonadal steroid profile and serum Vtg in A. altiparanae males, and their hepatic gene expression. In addition, somatic and morphological biomarkers will be evaluated. The research will offer new insights about the ecotoxicological relevance of pharmacological compounds in reproductive physiology of teleost. (AU)