Evaluation of antimicrobial and antibiofilm activities of extracts and fractions from plants against oral pathogenic microorganisms

• Grant number: 17/07408-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2017
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marlise Inêz Klein Furlan
• Grantee: Sabrina Marcela Ribeiro
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The increase of microbial resistance to known antimicrobials and the difficulty to eradicate microorganism growing in biofilms indicate a need for searching novel natural agents with antimicrobial and antibiofilm activities. A rich resource of these agents are plant extracts and fractions. In the oral cavity, biofilm formation initiates with planktonic microorganisms adhering to the salivary pellicle on teeth and with the formation of the initial extracellular matrix, which is primarily composed of glucans synthetized by glucosiltransferases. Therefore, the aim of this study is to determine the antimicrobial and antibiofilm effect of extracts and fractions from plant species (including different accessions of Casearia sylvestris) against oral pathogenic microorganisms. The study will have three major research steps: 1- Evaluation of antimicrobial (antibiotic and antifungal) potential of crude extracts and fractions from plant species via minimum inhibitory concentration (MIC) and minimum bactericidal and fungicidal concentration (MBC/MFC) tests; 2- Assessment of the antibiofilm effect of extracts and fractions from plant species by determining the biomass via violet crystal method and the microbial population via platting of treated biofilms; 3- Evaluation of the inhibition capability of extracts and fractions from plant species on the initial steps of biofilm formation; specifically, the effect of treatments on the glucan synthesis on saliva-coated hydroxyapatite surface and on the microbial adherence to the salivary pellicle and to the initial matrix (glucans) on formed hydroxyapatite surface. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; FERNANDES, JULIA M.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO JOSE; KLEIN, I, MARLISE. Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 169, MAR 2021. (14/50926-0, 13/07600-3, 12/21921-4, 17/07408-6, 19/23175-7, 11/21440-3)

CASTILLO PEDRAZA, MIDIAN C.; DE OLIVEIRA FRATUCELLI, ERICK DANTE; RIBEIRO, SABRINA MARCELA; FLOREZ SALAMANCA, ELKIN JAHIR; COLIN, JAQUELINE SILVA; KLEIN, I, MARLISE. Modulation of Lipoteichoic Acids and Exopolysaccharides Prevents Streptococcus mutans Biofilm Accumulation. Molecules, v. 25, n. 9, MAY 2020. (17/07408-6, 14/05423-0, 17/26623-5, 17/00753-0)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; BUENO, PAULA C. P.; DE CASTRO, V, MARLENE KELLY; FRANCISCO, AMANDA ALCALA; CAVALHEIRO, ALBERTO JOSE; KLEIN, I, MARLISE. Antimicrobial and antibiofilm activities of Casearia sylvestris extracts from distinct Brazilian biomes against Streptococcus mutans and Candida albicans. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 19, n. 1, NOV 12 2019. (13/07600-3, 14/50926-0, 11/21440-3, 12/21921-4, 17/07408-6)

RIBEIRO, SABRINA M.; FRATUCELLI, ERICK D. O.; FERNANDES, JULIA M.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO JOSE; KLEIN, MARLISE, I. Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v. N/A, n. 169, p. 23-pg., 2021-03-01. (19/23175-7, 13/07600-3, 17/07408-6, 11/21440-3, 12/21921-4, 14/50926-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RIBEIRO, Sabrina Marcela. Antimicrobial and antibiofilm activities of Casearia sylvestris extracts and fractions from distinct Brazilian biomes against Streptococcus mutans and Candida albicans. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.