Evaluation of the adhesion and proliferation processes of cellular in PEDOT-co-PDLLA thin films supported on Au electrodes functionalized with laminin, integrin and fibronectin

• Grant number: 17/08349-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2017
• End date: July 21, 2021
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Susana Inés Córdoba de Torresi
• Grantee: Rubens Araujo da Silva
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage, AP.TEM
• Associated scholarship(s): 18/18846-7 - Evaluation of adhesion and proliferation processes of cellular in PEDOT-co-PDLLA thin films using electrical stimulating regimes and mechanical force application, BE.EP.PD

Abstract

Functional surface coatings are a key option for many biomedical applications, from polymeric supports for tissue engineering to smart matrices for controlled drugs delivery. Within this scenario, the synthesis of new materials for biological applications and the development of new processes are promising. For this proposal, biocompatible and electroactive polymers are interesting since they allow the study of cell adhesion and proliferation processes, especially when the polymer used has conductive properties. Despite these desirable features, it is well-known that most conductive polymers exhibit low degradability and have considerable cytotoxicity, limiting their applications in biotechnological routines. The PEDOT-co-PDLLA copolymer exhibits the physical-chemistry and mechanical characteristics required by bio-clinic interest, associated with electroactive, biocompatible and biodegradable properties. This copolymer can be applied to adhesion and proliferation cells, especially if functionalized with anchoring proteins (laminin, integrin and fibronectin). The adhesion cells can be subsequently subject electrical stimulated to cellular metabolism and proliferation increasing. The main advantage of using this copolymer is the possibility of eliminating the need for surgical intervention to remove it, as usually happens in some cases with metal implants, since the idea is the copolymer degradation at the same time that the bone tissue regenerates. It is expected the procedure to be developed will be accessible for application of this copolymer with conductive and biodegradable properties to tissue engineering in simple, low cost and routine bio-clinic situations

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, RUBENS ARAUJO; XUE, RUIKANG; DE TORRESI, SUSANA INES CORDOBA; CARTMELL, SARAH. Capacitive electrical stimulation of a conducting polymeric thin film induces human mesenchymal stem cell osteogenesis. BIOINTERPHASES, v. 17, n. 1, JAN 2022. (15/26308-7, 18/18846-7, 17/08349-3)

DOURADO, ANDRE H. B.; SILVA, RUBENS A.; TORRESI, ROBERTO M.; SUMODJO, PAULO T. A.; ARENZ, MATTHIAS; CORDOBA DE TORRESI, SUSANA I.. Kinetics, Assembling, and Conformation Control of L-Cysteine Adsorption on Pt Investigated by in situ FTIR Spectroscopy and QCM-D. ChemPhysChem, v. 19, n. 18, p. 2340-2348, SEP 18 2018. (17/08349-3, 13/25592-8, 15/08300-9)

DA SILVA, ARUA C.; DA SILVA, RUBENS A.; SOUZA, MARIA J. P. G.; MONTOYA, PAULA M.; BENTINI, RICARDO; AUGUSTO, TATIANA; TORRESI, ROBERTO M.; CATALANI, LUIZ H.; DE TORRESI, SUSANA I. CORDOBA. Electrochemical quartz crystal microbalance with dissipation investigation of fibronectin adsorption dynamics driven by electrical stimulation onto a conducting and partially biodegradable copolymer. BIOINTERPHASES, v. 15, n. 2, MAR 2020. (18/13492-2, 17/08349-3, 15/26308-7, 17/00705-5, 14/09353-6)