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Bacterial biosynthesis of indole acetic acid L-tryptophan dependent and influence on the modulation of vegetal growth

Grant number: 17/11026-1
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2017
Effective date (End): August 31, 2018
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Maria Carolina Quecine Verdi
Grantee:Everthon Fernandes Figueredo
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:14/16804-4 - Deciphering the molecular interaction between Puccinia psidii x Eucaliptus grandis trough "omics" approaches, AP.JP

Abstract

Among the mechanisms that originate from the bacterium-plant interaction, the bacterial biosynthesis of indole acetic acid (IAA) plays a fundamental role in promoting plant growth, affecting processes such as cell division and elongation, tropism, apical dominance, senescence, flowering and response to stress. Different metabolic pathways are used by bacteria for the biosynthesis of IAA, and the pathway IPyA is mainly regulated by the ipdC gene and whose precursor is the amino acid L-tryptophan, the most commonly described. Nevertheless, studies related to bacterial IAA biosynthesis are incipient. In this context, molecular studies about the metabolic pathways of bacterial synthesis of IAA and the genes involved in this process are preponderant to understand its influence on the interaction between plant-bacteria and plant growth modulation. The Bacillus sp. RZ2MS9, isolated from the rhizosphere of guaraná, has been showing satisfactory activity in the promotion of plant growth. The sequencing of its genome pointed to the presence of a wide range of genes related to growth promotion, especially genes encoding auxins. Thus, the present project aims at the study of the influence and function of the ipdC gene on IAA biosyntthesis via L-Tryptophan (L-Trp) dependent on plant growth promoter rhizobacterium (PGPR), Bacillus sp. RZ2MS9 strain, and in plant growth modulation. For this, the knockout of this gene will be performed by the CRISPR-Cas9 technique. The wild-type RZ2MS9 (wt) and knockout ipdC gene (”ipdC) lines will be evaluated for AIA biosynthesis from L-tryptophan via the metabolic pathway IPyA using liquid chromatography coupled to mass spectrometry (LC-MS). In addition, bioassays promoting plant growth and modulating root architecture by the rhizobacterium in the model plant, Solanum lycopersicum cv. Micro-Tom and its defective mutant for IAA receptor, ”dgt will be performed to elucidate and characterize the physiologically IAA bacterial paper in plants. Certainly, these studies provide a better understanding of the role of RZ2MS9 bacterial IAA biosynthesis L-Trp dependent in the promotion of plant growth, in order to obtain more efficient responses in its biotechnological application as a bioinoculant in agricultural crops.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, ISANELI BATISTA; LOPES, MARIANA DA SILVA; BINI, ANDRESSA PERES; PROHMANN TSCHOEKE, BRUNO AUGUSTO; WRUCK VERSSANI, BRUNA APARECIDA; FIGUEREDO, EVERTHON FERNANDES; CATALDI, THAIS REGIANI; RODRIGUES MARQUES, JOAO PAULO; SILVA, LUCIANA DUQUE; LABATE, CARLOS ALBERTO; QUECINE, MARIA CAROLINA. The Eucalyptus Cuticular Waxes Contribute in Preformed Defense Against Austropuccinia psidii. FRONTIERS IN PLANT SCIENCE, v. 9, JAN 9 2019. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FIGUEREDO, Everthon Fernandes. Knockout of the ipdC gene in Bacillus sp. (RZ2MS9) with a CRISPR-Cas9 and influence on the IAA biosynthesis L-tryptophan dependent. 2018. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz Piracicaba.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.