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Ecotoxicological study of the anti-fouling biocide Sea-Nine in neotropical marine invertebrates.

Grant number: 17/10211-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2017
Effective date (End): December 31, 2021
Field of knowledge:Physical Sciences and Mathematics - Oceanography - Biological Oceanography
Principal Investigator:Denis Moledo de Souza Abessa
Grantee:Bruno Galvão de Campos
Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil
Associated scholarship(s):19/19898-3 - Trophic transfer assessment of the antifouling biocide DCOIT, in nano and non-nanostructured forms, in marine organisms, BE.EP.DR


The biocide Sea-Nine (4,5-dichloro-2-n-octyl-4-isothiazolin-3-one) has been widely used as active principle in third generation antifouling paints, since the Tributyltin (TBT) based coatings were banned. There are few studies on the toxicity of the Sea-Nine to non-target organisms as well as its ecological risks; moreover, its mechanisms of action (at cellular and biochemical levels) are still not fully understood. This project aims to assess the toxicity of Sea-Nine to marine invertebrates, as well as investigate its key mechanisms of action, in order to provide some information on the ecological risks related to this biocide. To accomplish that, aqueous solutions and spiked sediments contaminated with Sea-Nine will be evaluad by means of acute or chronic toxicity tests using the following organisms: Nitocra sp (Copepod), Tiburonella viscana (amphipod), Kalliapseudes schubartii (tanaidaceous), Perna perna (bivalve) embryos, Echinometra lucunterm (sea-urchin), and Artemia sp (Anostraca). The mechanisms of action at the cellular and biochemical levels will be analyzed through biochemical and cellular biomarkers (GST, GSH, GPx, Lipoperoxidation, DNA damage, ATPases, Caspases and neutral red retention assay) using the bivalve Perna perna exposed to environmentally relevant concentrations of the Sea-Nine. This proposal is innovative because it is the first study considering the toxic effects of the Sea-Nine to non-target neotropical organisms. The results will provide important information on the effects and ecological of the Sea-Nine, which could support biomonitoring actions and the establishment of environmental laws and enforcements.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CAMPOS, BRUNO GALVAO; DO PRADO E SILVA, MARIANA BRUNI MARQUES; AVELELAS, FRANCISCO; MAIA, FREDERICO; LOUREIRO, SUSANA; PERINA, FERNANDO; DE SOUZA ABESSA, DENIS MOLEDO; MARTINS, ROBERTO. Toxicity of innovative antifouling additives on an early life stage of the oyster Crassostrea gigas: short- and long-term exposure effects. Environmental Science and Pollution Research, . (20/03004-0, 19/19898-3, 18/25379-6, 17/10211-0, 19/24945-0)
DE CAMPOS, BRUNO GALVAO; FONTES, MAYANA KAROLINE; GUSSO-CHOUERI, PALOMA KACHEL; MARINSEK, GABRIELA PUSTIGLIONE; NOBRE, CAIO RODRIGUES; MORENO, BEATRIZ BARBOSA; LEMOS ABREU, FIAMMA EUGENIA; FILLMANN, GILBERTO; MARI, RENATA DE BRITTO; DE SOUZA ABESSA, DENIS MOLEDO. preliminary study on multi-level biomarkers response of the tropical oyster Crassostrea brasiliana to exposure to the antifouling biocide DCOI. Marine Pollution Bulletin, v. 174, . (17/10211-0, 17/04970-5, 20/03004-0)
NICOLAU DOS SANTOS, JULIANA VITORIA; MARTINS, ROBERTO; FONTES, MAYANA KAROLINE; DE CAMPOS, BRUNO GALVAO; SILVA, MARIANA BRUNI MARQUES DO PRADO E.; MAIA, FREDERICO; DE SOUZA ABESSA, DENIS MOLEDO; PERINA, FERNANDO CESAR. Can Encapsulation of the Biocide DCOIT Affect the Anti-Fouling Efficacy and Toxicity on Tropical Bivalves?. APPLIED SCIENCES-BASEL, v. 10, n. 23, . (18/25379-6, 19/19898-3, 17/10211-0, 16/24033-3)
DE CAMPOS, BRUNO GALVAO; FIGUEIREDO, JOANA; PERINA, FERNANDO; ABESSA, DENIS MOLEDO DE SOUZA; LOUREIRO, SUSANA; MARTINS, ROBERTO. Occurrence, effects and environmental risk of antifouling biocides (EU PT21): Are marine ecosystems threatened?. CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY, v. 52, n. 18, p. 32-pg., . (17/10211-0, 19/19898-3)
CAMPOS, B. G.; MOREIRA, L. B.; PAULY, G. F. E.; CRUZ, A. C. F.; PERINA, F. C.; ABREU, F.; FILLMANN, G.; ABESSA, D. M. S.. Water and sediment toxicity and hazard assessment of DCOIT towards neotropical marine organisms. Environmental Pollution, v. 330, p. 9-pg., . (16/02029-4, 21/06167-0, 20/00068-8, 20/03004-0, 21/08471-9, 18/23279-4, 17/10211-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CAMPOS, Bruno Galvão de. Ecotoxicological study of the antifouling biocide DCOIT (SEA-NINE™) in Neotropical marine invertebrates. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. São Vicente São Vicente.

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