Whole-genome sequencing approach to study multidrug-resistant Salmonella enterica isolates from Brazilian slaughterhouse

• Grant number: 17/15967-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2017
• End date: September 30, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Mariza Landgraf
• Grantee: Daniel Farias Marinho Do Monte
• Supervisor: Paula J. Fedorka-Cray
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: North Carolina State University (NC State), United States
• Associated to the scholarship: 16/03044-7 - Genetic diversity, virulence genes and antimicrobial resistance of Salmonella spp. isolated from poultry slaughterhouse, BP.DR

Abstract

The characterization of foodborne pathogen has changed over the years, although traditional typing systems based on phenotypic characteristics, such as antibiogram, biotype and serotype are still widely used. The advent of molecular biology and the use of techniques based on DNA analysis have revolutionized the field of epidemiology and pathogen characterization and whole genome sequencing (WGS) allows scientists and industries generate dataset quickly to solve problems. Bioinformatics can be used as a useful tool in the epidemiological investigation of Salmonella distributed in poultry production chain and this pathogen can now be examined in silico for the presence of antimicrobial resistance (AMR) and virulence genes immediately after sequencing, while traditional AMR testing is time-consuming and expensive. Considering this aspects, the present project aims to investigate the antimicrobial resistance, virulence and genotypic diversity of Salmonella serotypes important in Brazilian chicken production applying whole genome sequencing approaches. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MONTE, DANIEL F. .; LINCOPAN, NILTON; BERMAN, HANNA; CERDEIRA, LOUISE; KEELARA, SHIVARAMU; THAKUR, SIDDHARTHA; FEDORKA-CRAY, PAULA J.; LANDGRAF, MARIZA. Genomic Features of High-Priority Salmonella enterica Serovars Circulating in the Food Production Chain, Brazil, 2000-2016. SCIENTIFIC REPORTS, v. 9, JUL 30 2019. (13/07914-8, 17/15967-5, 16/03044-7)

MONTE, DANIEL F.; LINCOPAN, NILTON; FEDORKA-CRAY, PAULA J.; LANDGRAF, MARIZA. Current insights on high priority antibiotic-resistant Salmonella enterica in food and foodstuffs: a review. CURRENT OPINION IN FOOD SCIENCE, v. 26, p. 12-pg., 2019-04-01. (16/03044-7, 13/07914-8, 17/15967-5)

MONTE, DANIEL F. M.; NETHERY, MATTHEW A.; BARRANGOU, RODOLPHE; LANDGRAF, MARIZA; FEDORKA-CRAY, PAULA J.. Whole-genome sequencing analysis and CRISPR genotyping of rare antibiotic-resistant Salmonella enterica serovars isolated from food and related sources. FOOD MICROBIOLOGY, v. 93, p. 9-pg., 2021-02-01. (13/07914-8, 17/15967-5)

MONTE, DANIEL F. M.; FUGA, BRUNA; PARIONA, JESUS G. M.; VASQUEZ-PONCE, FELIPE; BECERRA, JOHANA; KEELARA, SHIVARAMU; THAKUR, SIDDHARTHA; FEDORKA-CRAY, PAULA; LINCOPAN, NILTON; LANDGRAF, MARIZA. Highly virulent colistin-susceptible Salmonella Havana ST1524 carrying the mcr-9.1 gene in food. Microbial Pathogenesis, v. 199, p. 6-pg., 2024-12-27. (16/03044-7, 13/07914-8, 17/15967-5)

MONTE, DANIEL F.; LINCOPAN, NILTON; CERDEIRA, LOUISE; FEDORKA-CRAY, PAULA J.; LANDGRAF, MARIZA. Early Dissemination of qnrE1 in Salmonella enterica Serovar Typhimurium from Livestock in South America. Antimicrobial Agents and Chemotherapy, v. 63, n. 9, SEP 2019. (13/07914-8, 17/15967-5, 16/03044-7)

MONTE, DANIEL F. M.; NETHERY, MATTHEW A.; BERMAN, HANNA; KEELARA, SHIVARAMU; LINCOPAN, NILTON; FEDORKA-CRAY, PAULA J.; BARRANGOU, RODOLPHE; LANDGRAF, MARIZA. Clustered Regularly Interspaced Short Palindromic Repeats Genotyping of Multidrug-Resistant Salmonella Heidelberg Strains Isolated From the Poultry Production Chain Across Brazil. FRONTIERS IN MICROBIOLOGY, v. 13, p. 14-pg., 2022-06-17. (17/15967-5, 16/03044-7, 13/07914-8)