Generation of induced pluripotent stem cells (iPSCs) through different reprogramming methods and characterization of pluripotency state in the dog model.

• Grant number: 17/14884-9
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: January 05, 2018
• End date: July 04, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: André Furugen Cesar de Andrade
• Grantee: Naira Caroline Godoy Pieri
• Supervisor: Poul Hyttel
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Copenhagen, Frederiksberg, Denmark
• Associated to the scholarship: 15/25564-0 - In vitro generation of porcine primordial germ cells and functional evaluation in vivo., BP.PD

Abstract

In 2006, Shinya Yamanaka's group reported that somatic cells could be reprogrammed into pluripotent stem cells (induced pluripotent cells, iPSCs) by retroviral overexpression of four specific transcription factors: OCT4, C-MYC, SOX2 and KLF4 (OMSK). Since then, the establishment of iPS cell technology has been through a great development in the field of biology and medical science. Much effort has been invested in revealing the pathways leading to reactivation of the endogenous pluripotency apparatus in differentiated cells, and in identifying factors that regulate this process. Recent efforts focused on developing technologies to derive transgene-free iPS cells with the aim of obtaining cells that are safe, robust and reproducible. However many have investigated the reprogramming of mouse and human somatic cells into iPS cells, but few reports have emerged on successful reprogramming of differentiated somatic cell-derived induced pluripotency stem cells (iPS) in domestic species, for example, canine and swine. Indeed, both species are considered adequate models for human medicine. Therefore, this study aims to understand the underlying mechanisms that lead to canine induced pluripotency stem cells (ciPS) with two different reprogramming techniques: the use of integrative (lentiviral) or not (episomal) vectors; as well as to characterize the produced cells regarding their pluripotency. It is expected that the results obtained herein may elucidate the biotechnological potential of these cells to be further used in disease modeling.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GODOY PIERI, NAIRA CAROLINE; DE SOUZA, ALINE FERNANDA; BOTIGELLI, RAMON CESAR; DE FIGUEIREDO PESSOA, LAIS VICARI; RECCHIA, KAIANA; MACHADO, LUCAS SIMOES; GLORIA, MAYRA HIRAKAWA; GUIMARAES DE CASTRO, RAQUEL VASCONCELOS; LEAL, DIEGO FEITOSA; NETO, PAULO FANTINATO; et al. Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions. STEM CELL REVIEWS AND REPORTS, JUN 2021. (13/08135-2, 15/26818-5, 15/25564-0, 17/14884-9)

GODOY PIERI, NAIRA CAROLINE; DE SOUZA, ALINE FERNANDA; BOTIGELLI, RAMON CESAR; DE FIGUEIREDO PESSOA, LAIS VICARI; RECCHIA, KAIANA; MACHADO, LUCAS SIMOES; GLORIA, MAYRA HIRAKAWA; GUIMARAES DE CASTRO, RAQUEL VASCONCELOS; LEAL, DIEGO FEITOSA; NETO, PAULO FANTINATO; et al. Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions. STEM CELL REVIEWS AND REPORTS, v. 18, n. 5, p. 18-pg., 2021-06-11. (13/08135-2, 15/26818-5, 15/25564-0, 17/14884-9)